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Team:Cambridge/BioluminescenceLinks
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==Emily's Section== | ==Emily's Section== | ||
*Spectra: | *Spectra: | ||
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* Some actual numbers on luciferin [http://pubs.acs.org/doi/abs/10.1021/bi060475o] | * Some actual numbers on luciferin [http://pubs.acs.org/doi/abs/10.1021/bi060475o] | ||
* D-cysteine is bad for e-coli [http://www.jbc.org/content/276/44/40864.full] | * D-cysteine is bad for e-coli [http://www.jbc.org/content/276/44/40864.full] | ||
| + | * luminaze - good for brighter and longer wavelength light [http://pubs.acs.org/doi/abs/10.1021/bi00327a026] | ||
==Will's section== | ==Will's section== | ||
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==Ben's section== | ==Ben's section== | ||
| - | * Flashlight fish symbiont luciferase genes have been sequenced but cannot be | + | * Flashlight fish symbiont luciferase genes have been sequenced but cannot be successfully expressed in E. Coli |
* Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/ | * Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/ | ||
* The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html | * The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html | ||
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[[Image:luciferin.png|600px]] | [[Image:luciferin.png|600px]] | ||
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| + | * L-luciferin can be oxidised to produce light but with half the efficiency http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1217473/ | ||
==Paul's section== | ==Paul's section== | ||
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Latest revision as of 23:45, 10 August 2010
Rough notes: Bioluminescence
Emily's Section
- Spectra:
- Spectral Emissions of different species - Different Species Spectra
- Fluorescent Protein Spectra - Spectra
- Small amount of blue-shift - Blue Shift
- More blue-shift - Blue Shift 2
- Metridia: (luciferase requires CBP Protein)
- Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048
- CBP link - http://www.ncbi.nlm.nih.gov/pubmed/18264586
- CBP amino acid sequence http://www.rsc.org/ej/PP/2008/b713109g.pdf
- Firefly:
- Oxyluciferin reduction - http://www.ncbi.nlm.nih.gov/pubmed/19872330
- Luciferin Regenerating Enzyme (LRE) - LRE (in Photinus pyralis)
- Firefly luciferin reaction - http://pubs.acs.org/doi/pdf/10.1021/ja00529a051
- Light Emission Quanta - Spectral Emission and Quantum Yield of Firefly Bioluminescence
- One light quantum emitted for every luciferin molecule oxidized
- CoA stimulation - CoA in Luminescence
- D-cysteine powerfully inhibits E.Coli growth D-cysteine
- Aequorin:
- Regeneration of Aequorin in Aequorea Aequorea jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf
- Bacteria:
- Bacterial luciferase reaction - Bacterial Luciferin
- Beetle luciferin - Putative Beetle Luciferin Synthesis
- V. Fischeri Plasmid - V. Fischeri Plasmid
- KCal/Mol luciferin in Lightning Bugs - Luciferin KCal/Mol
- Photinus Pyralis (firefly) spectrum, 562nm - Reference
- Different Coloured Firefly Luciferases - Colours
- Gaussia Princeps (copepod):
Theo's section
- Self contained part for Photorhabdus luminescens lux operon: http://partsregistry.org/Part:BBa_J70008:Design
- Firefly diagram from Emily's paper:
- Fireflow luciferin normally emits at 560nm but at higher temperatures at 630nm
- http://pubs.acs.org/doi/pdf/10.1021/bi00702a015 Kinetics
- Mg2+ required
- A nice background summary: http://irishmarinelife.com/bioluminescence-symbioses/
- The guy who made the biobrick's logbook: http://openwetware.org/wiki/User:Spenser_Skates/Notebook/pTKX_luciferase_biobrick/2008/04/14
Vibrio
- luxC = acyl coA reductase
Firefly
- The awesome paper http://www.springerlink.com/content/67w1q80435537747/fulltext.pdf
Luciferases=
- Coelentrazine using
- Firefly luciferin using
- Produces all parts of pathway:
- Click beetle:
Fluorescent proteins
Bill's section
- Conversion of normal luciferin into firefly luciferin
- Don't use luciferinol, it r teh suckage
- Glowing fish what?
- Some actual numbers on luciferin [1]
- D-cysteine is bad for e-coli [2]
- luminaze - good for brighter and longer wavelength light [3]
Will's section
Anja's section
- Paper on mutant luciferase with increased activity
- Aequorin wavelength= 469 nm
- Low leakage rate
- Does not disrupt cell functions/ devt.
- Requires continual addition of luciferin
- Dr Laura Rowe's Paper on Bioluminescent Proteins
- Paper on Biosynthesis of Firefly D-Luciferin
- Paper on L-Tryptophan in Biosynthesis of Sea Firefly Luciferase
Peter's section
Ben's section
- Flashlight fish symbiont luciferase genes have been sequenced but cannot be successfully expressed in E. Coli
- Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/
- The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html
- Firefly luciferin biosynthesis appears not to be known. Vibrio Ficheri Lux operon is known. Would VF luciferin work with other luciferases? http://www3.interscience.wiley.com/cgi-bin/fulltext/89014035/PDFSTART
- L-luciferin can be oxidised to produce light but with half the efficiency http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1217473/
Paul's section
- Precursor to luciferin: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=261858|ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
- Construction of a new firefly bioluminescence system using l-luciferin as substrate
- stuff to do with ATP in the cycle
- Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase
- Biobrick for the Firefly luciferase for Slovenia '07: http://partsregistry.org/Part:BBa_I712019:Design
- Molecular cloning and expression of the cDNAs encoding luciferin-regenerating enzyme from Luciola cruciata and Luciola lateralis
