Team:Cambridge/BioluminescenceLinks
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==Paul's section== | ==Paul's section== | ||
* Precursor to luciferin: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=261858|ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC | * Precursor to luciferin: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=261858|ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC | ||
+ | * [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THS-4HWX8NX-C&_user=1495569&_coverDate=02%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402624057&_rerunOrigin=google&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=b2ccca6fb4c15f90120534c45c40bc8e Construction of a new firefly bioluminescence system using l-luciferin as substrate] | ||
+ | * [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-4DYN47Y-NX&_user=1495569&_origUdi=B6T21-3XX6W2J-18&_fmt=high&_coverDate=08%2F01%2F1992&_rdoc=1&_orig=article&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=84b46718e73359df8c6aea608dde9f15 stuff to do with ATP in the cycle] | ||
+ | * [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THS-4HWX8NX-C&_user=1495569&_coverDate=02%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402624057&_rerunOrigin=google&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=b2ccca6fb4c15f90120534c45c40bc8e Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase] | ||
{{:Team:Cambridge/Templates/footer}} | {{:Team:Cambridge/Templates/footer}} |
Revision as of 14:33, 16 July 2010
Bioluminescence
Emily's Section
- Metridia luciferase - requires CBP Protein
- Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048
- CBP link - http://www.ncbi.nlm.nih.gov/pubmed/18264586
- CBP amino acid sequence http://www.rsc.org/ej/PP/2008/b713109g.pdf
- Oxyluciferin reduction - http://www.ncbi.nlm.nih.gov/pubmed/19872330
- Luciferin Regenerating Enzyme (LRE) - LRE (in Photinus pyralis)
- Regeneration of Aequorin in Aequorea Aequorea jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf
- Firefly luciferin reaction - http://pubs.acs.org/doi/pdf/10.1021/ja00529a051
- Light Emission Quanta - Spectral Emission and Quantum Yield of Firefly Bioluminescence
- One light quantum emitted for every luciferin molecule oxidized
- CoA stimulation - CoA in Luminescence
- Bacterial luciferase reaction - Bacterial Luciferin
- Beetle luciferin - Putative Beetle Luciferin Synthesis
- V. Fischeri Plasmid - V. Fischeri Plasmid
- KCal/Mol luciferin in Lightning Bugs - Luciferin KCal/Mol
- Spectral Emissions of different species - Different Species Spectra
- Photinus Pyralis (firefly) spectrum, 562nm - Reference
- Fluorescent Protein Spectra - Spectra
- Different Coloured Firefly Luciferases - Colours
- Small amount of blue-shift - Blue Shift
Theo's section
- Self contained part for Photorhabdus luminescens lux operon: http://partsregistry.org/Part:BBa_J70008:Design
- Firefly diagram from Emily's paper:
- Fireflow luciferin normally emits at 560nm but at higher temperatures at 630nm
- http://pubs.acs.org/doi/pdf/10.1021/bi00702a015 Kinetics
- Mg2+ required
- A nice background summary: http://irishmarinelife.com/bioluminescence-symbioses/
- The guy who made the biobrick's logbook: http://openwetware.org/wiki/User:Spenser_Skates/Notebook/pTKX_luciferase_biobrick/2008/04/14
Vibrio
- luxC = acyl coA reductase
Firefly
- The awesome paper http://www.springerlink.com/content/67w1q80435537747/fulltext.pdf
Bill's section
- Conversion of normal luciferin into firefly luciferin
- Don't use luciferinol, it r teh suckage
- Glowing fish what?
Will's section
Anja's section
- Paper on mutant luciferase with increased activity
- Aequorin wavelength= 469 nm
- Low leakage rate
- Does not disrupt cell functions/ devt.
- Requires continual addition of luciferin
- Dr Laura Rowe's Paper on Bioluminescent Proteins
- Paper on Biosynthesis of Firefly D-Luciferin
- Paper on L-Tryptophan in Biosynthesis of Sea Firefly Luciferase
Peter's section
Ben's section
- Flashlight fish symbiont luciferase genes have been sequenced but cannot be sucessfully expressed in E. Coli
- Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/
- The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html
- Firefly luciferin biosynthesis appears not to be known. Vibrio Ficheri Lux operon is known. Would VF luciferin work with other luciferases? http://www3.interscience.wiley.com/cgi-bin/fulltext/89014035/PDFSTART
Paul's section
- Precursor to luciferin: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=261858|ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC
- Construction of a new firefly bioluminescence system using l-luciferin as substrate
- stuff to do with ATP in the cycle
- Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase