Team:Cambridge/Bioluminescence/Vibrio Characterisation

From 2010.igem.org

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=Effect of pH=
 
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Both the intensity and the spectrum emitted by the luciferase-luciferin reaction has been shown to be higly dependent on the pH of the medium. The main characterisation experiments have been performed in LB Broth at pH 7, so in order to assess this effect cultures with LB and a citrate buffer were prepared (pH = 5.3, pH 6.1 and pH = 7) This page describes the results of these experiments. We used a [http://www.bmglabtech.com/products/microplate-reader/instruments.cfm?product_id=2 FLUOstar OPTIMA] microplate reader to quantify the light output. Protocols and plate reader settings used are given below.
 
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{{:Team:Cambridge/Templates/Nolineheader|header=Data}}
 
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'''Maximum light output within 5 hours of D-luciferin injection at different pH values.'''
 
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[http://partsregistry.org/wiki/images/e/e8/Phhistogram.png http://partsregistry.org/wiki/images/thumb/e/e8/Phhistogram.png/569px-Phhistogram.png]
 
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These values are the mean of 3 readings. The corresponding error bars represent an interval of twice the standard deviation across the 3 data points centred around the mean value.
 
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'''Evolution of light output at different values of pH.'''
 
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[http://partsregistry.org/wiki/images/d/d8/Phtimecourse.png http://partsregistry.org/wiki/images/thumb/d/d8/Phtimecourse.png/569px-Phtimecourse.png]
 
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Measurements are taken every 20 min. These values are the mean of 3 readings. The corresponding error bars represent an interval of twice the standard deviation across the 3 data points centred around the mean value.
 
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{|{{Table}}
 
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!Data
 
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!Notes
 
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!Date Uploaded
 
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|[http://partsregistry.org/wiki/images/6/64/BBa_K325219pheffect.xls Media:BBa_K325219pheffect.xls]
 
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|Raw data from experiment
 
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|21/10/2010
 
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{{:Team:Cambridge/Templates/Nolineheader|header=Protocol}}
 
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#The protocol can be found as [http://2010.igem.org/Team:Cambridge/Notebook/Week11 Experiment 110].
 
=Compatibility=
=Compatibility=

Revision as of 21:07, 27 October 2010