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Team:Cambridge/Bioluminescence/Firefly Modelling
From 2010.igem.org
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{{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Modelling}} | {{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Modelling}} | ||
| - | We modelled the Luciferin cycle as described | + | We modelled the Luciferin cycle as described by [http://www.ncbi.nlm.nih.gov/pubmed/18949818 Marques 2009 and others. We were assisted by data from [http://www.ncbi.nlm.nih.gov/pubmed/19859663 S. Inouye (2010)] and [http://www.ncbi.nlm.nih.gov/pubmed/20655239 J.M Leitão ''et al.'' (2010)] on the inhibition of firefly luciferase. |
We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below: | We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below: | ||
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[[Image:Modelluccycle.jpg|center|700px]] | [[Image:Modelluccycle.jpg|center|700px]] | ||
| - | Some | + | Some parameters were taken from literature. However there was no clear data for others, such that we were only able to define some parameters in the light of experimental data. |
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| + | While attempting to optimise our system we modelled the effect of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration Luciferin Regenerating Enzyme (LRE)] | ||
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We modelled the system both with and without the effect of the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration Luciferase Regenerating Enzyme] (LRE.) The 4 Figures below show a comparison the model and our data for both cases. | We modelled the system both with and without the effect of the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration Luciferase Regenerating Enzyme] (LRE.) The 4 Figures below show a comparison the model and our data for both cases. | ||
| + | [[Image:Cambridge-LREmodel.png|thumb|center|700px|'''Figure 1 - Model of the light output as a function of time without the effect of the LRE. ''']] | ||
| + | Our model suggested that the LRE would make light output increase faster, peak higher and decay more slowly. These were all qualities which suited us. | ||
[[Image:Modellight.png|thumb|center|700px|'''Figure 1 - Model of the light output as a function of time without the effect of the LRE. ''']] | [[Image:Modellight.png|thumb|center|700px|'''Figure 1 - Model of the light output as a function of time without the effect of the LRE. ''']] | ||
Revision as of 01:57, 27 October 2010
We modelled the Luciferin cycle as described by Marques 2009 and others. We were assisted by data from [http://www.ncbi.nlm.nih.gov/pubmed/19859663 S. Inouye (2010) and J.M Leitão et al. (2010) on the inhibition of firefly luciferase.
We used the Matlab Simbiology toolbox to model the cycle. A diagram of the model we developed can be found below:
Some parameters were taken from literature. However there was no clear data for others, such that we were only able to define some parameters in the light of experimental data.
While attempting to optimise our system we modelled the effect of Luciferin Regenerating Enzyme (LRE)
We modelled the system both with and without the effect of the Luciferase Regenerating Enzyme (LRE.) The 4 Figures below show a comparison the model and our data for both cases.
Our model suggested that the LRE would make light output increase faster, peak higher and decay more slowly. These were all qualities which suited us.
