Team:Cambridge/Bioluminescence/Firefly Characterisation

From 2010.igem.org

(Difference between revisions)
(Compatibility)
 
(6 intermediate revisions not shown)
Line 3: Line 3:
This page describes characterisation for part [http://partsregistry.org/Part:BBa_K325219 BBa_K325219], Red luciferase and LRE operon from L. cruciata under pBAD.
This page describes characterisation for part [http://partsregistry.org/Part:BBa_K325219 BBa_K325219], Red luciferase and LRE operon from L. cruciata under pBAD.
-
It also gives a comparison of the light ouput of the different coloroured mutants we produced.  
+
It also gives a comparison of the light ouput of the different [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour coloured mutants] we produced.  
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Description|Description]]
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Description|Description]]
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Arabinose_to_light|Arabinose to light]]
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Arabinose_to_light|Arabinose to light]]
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Luciferin_to_light|Luciferin to light]]
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Luciferin_to_light|Luciferin to light]]
-
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Effect_of_pH|Effect of pH
+
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Effect_of_pH|Effect of pH]]
-
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Colour_Mutations|Coloured outputs]]
+
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Coloured_outputs|Coloured outputs]]
=Description=
=Description=
-
{{:Team:Cambridge/Templates/RightImage|image=Cambridge-low.jpg|caption=E.Coli (Invitrogen TOP 10) cells transformed with [http://partsregistry.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [http://partsregistry.org/Part:BBa_K325219 BBa 325219] (red light bulb)}}
+
{{:Team:Cambridge/Templates/RightImage|image=Redluc.jpg|caption=E.Coli (Invitrogen TOP 10) cells transformed with [http://partsregistry.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [http://partsregistry.org/Part:BBa_K325219 BBa 325219] (red light bulb)}}
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.
Line 118: Line 118:
In this section we compare the intensity of the different coloured mutants we developed during our project.
In this section we compare the intensity of the different coloured mutants we developed during our project.
 +
 +
{{:Team:Cambridge/Templates/Nolineheader|header=Data}}
[[Image:Outputpower.jpg|thumb|569px|center|'''Figure 1 - Maximum light output within 5 hours of D-Luciferin injection of the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour coloured mutants] we developed. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats.  ''']]
[[Image:Outputpower.jpg|thumb|569px|center|'''Figure 1 - Maximum light output within 5 hours of D-Luciferin injection of the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour coloured mutants] we developed. Data points and error bars correspond to the mean and the standard deviation of 3 time repeats.  ''']]
Line 126: Line 128:
!Date Uploaded
!Date Uploaded
|-
|-
-
|[[CambridgeiGEMcolouredoutputs.xls]]
+
|[[Media:CambridgeiGEMcolouredoutputs.xls]]
|Raw data from experiment
|Raw data from experiment
|21/10/2010
|21/10/2010
|}
|}
-
</center>
 
=Compatibility=
=Compatibility=
-
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''
+
Chassis: Device has been shown to work in ''Top 10 (Invitrogen)''
-
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
+
 
 +
Plasmids: Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br>
=References=
=References=

Latest revision as of 03:50, 28 October 2010