Team:Cambridge/Bioluminescence/Firefly Characterisation

From 2010.igem.org

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(Description)
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*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Coloured_outputs|Coloured outputs]]
*[[Team:Cambridge/Bioluminescence/Firefly_Characterisation#Coloured_outputs|Coloured outputs]]
=Description=
=Description=
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{{:Team:Cambridge/Templates/RightImage|image=Cambridge-low.jpg|caption=E.Coli (Invitrogen TOP 10) cells transformed with [http://partsregistry.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [http://partsregistry.org/Part:BBa_K325219 BBa 325219] (red light bulb)}}
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{{:Team:Cambridge/Templates/RightImage|image=Redluc.jpg|caption=E.Coli (Invitrogen TOP 10) cells transformed with [http://partsregistry.org/Part:BBa_K325909 BBa K325909] (blue light bulb) and [http://partsregistry.org/Part:BBa_K325219 BBa 325219] (red light bulb)}}
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.

Revision as of 03:42, 28 October 2010