Team:Cambridge/Bioluminescence/Colour

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{{:Team:Cambridge/Templates/headerMinimalprototype}}{{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Coloured outputs}}
{{:Team:Cambridge/Templates/headerMinimalprototype}}{{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Coloured outputs}}
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{{:Team:Cambridge/Templates/RightImage|image=Cambridge-Mutagenesis.jpg|caption=Comparison of the colour of Luciola cruciata we ordered from DNA 2.0 and it's colour after a single amino acid substitution}}
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{{:Team:Cambridge/Templates/RightImage|image=Rainbow.png|caption=Comparison of the different coloured outputs produced over the course of the project}}
The peak emission wavelength of luciola cruciata luciferase can be altered by single amino acid changes as reported by [http://www.ncbi.nlm.nih.gov/pubmed/1946326 Kajiyama and Nakano]. Different coloured outputs, as well as being aesthetically pleasing, allow the most appropriate wavelength of output for a particular detector to be chosen. By putting different coloured luciferases under promoters which respond to different inputs, co-reporter assays would be possible.
The peak emission wavelength of luciola cruciata luciferase can be altered by single amino acid changes as reported by [http://www.ncbi.nlm.nih.gov/pubmed/1946326 Kajiyama and Nakano]. Different coloured outputs, as well as being aesthetically pleasing, allow the most appropriate wavelength of output for a particular detector to be chosen. By putting different coloured luciferases under promoters which respond to different inputs, co-reporter assays would be possible.
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==Methods==
==Methods==
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We have created biobricks for Luciola cruciata luciferases of five different colours.   
We have created biobricks for Luciola cruciata luciferases of five different colours.   
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{| class="wikitable"
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<html>
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|-
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<table class="wikitable">
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| Part
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| Mutation
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<tr>
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| Colour
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<td width="100"> <b>Part</b>
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| λmax(nm)
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</td><td  width="100"> <b>Mutation</b>
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|-
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</td><td  width="100"> <b>Colour</b>
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| [http://partsregistry.org/Part:BBa_K325229 BBa_K325229
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</td><td  width="100"> <b>λmax(nm)</b>
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| Val239Ile
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</td></tr>
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| Green
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<tr>
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| 558
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<td> <a href="http://partsregistry.org/Part:BBa_K325229" class="external text" title="http://partsregistry.org/Part:BBa_K325229" rel="nofollow">BBa_K325229</a>
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|-
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</td><td> Val239Ile
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| [http://partsregistry.org/Part:BBa_K325209 BBa_K325209]
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</td><td> Green
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| WT
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</td><td> 558
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| Yellow/green  
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</td></tr>
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| 562
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<tr>
-
|-
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<td> <a href="http://partsregistry.org/Part:BBa_K325209" class="external text" title="http://partsregistry.org/Part:BBa_K325209" rel="nofollow">BBa_K325209</a>
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| [http://partsregistry.org/Part:BBa_K325219 BBa_K325219]
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</td><td> WT
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| Ser286Asn
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</td><td> Yellow/green
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| Red/Orange
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</td><td> 562
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| 607
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</td></tr>
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|-
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<tr>
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| [http://partsregistry.org/Part:BBa_K325239 BBa_K325239]
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<td> <a href="http://partsregistry.org/Part:BBa_K325219" class="external text" title="http://partsregistry.org/Part:BBa_K325219" rel="nofollow">BBa_K325219</a>
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| Gly326Ser
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</td><td> Ser286Asn
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| Scarlet
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</td><td> Red/Orange
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| 609
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</td><td> 607
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|-
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</td></tr>
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| [http://partsregistry.org/Part:BBa_K325249 BBa_K325249]
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<tr>
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| His433Ser
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<td> <a href="http://partsregistry.org/Part:BBa_K325239" class="external text" title="http://partsregistry.org/Part:BBa_K325239" rel="nofollow">BBa_K325239</a>
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| Red
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</td><td> Gly326Ser
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| 612
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</td><td> Scarlet
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|}
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</td><td> 609
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</td></tr>
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<tr>
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<td> <a href="http://partsregistry.org/Part:BBa_K325249" class="external text" title="http://partsregistry.org/Part:BBa_K325249" rel="nofollow">BBa_K325249</a>
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</td><td> His433Ser
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</td><td> Red
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</td><td> 612
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</td></tr>
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<tr>
 +
<td> <a href="http://partsregistry.org/Part:BBa_K325239" class="external text" title="http://partsregistry.org/Part:BBa_K325239" rel="nofollow">BBa_K325259</a>
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</td><td> Pro452Ser
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</td><td> Yellow
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</td><td> 595
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</td></tr></table>
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</html>
The bricks above are the luciferases with the Luciola cruciata luciferin regenerating enzyme under the control of an arabinose inducible promoter. The red/orange Ser286Asn mutant luciferase was also submitted with a promoter but no LRE and without a promoter or the LRE.  
The bricks above are the luciferases with the Luciola cruciata luciferin regenerating enzyme under the control of an arabinose inducible promoter. The red/orange Ser286Asn mutant luciferase was also submitted with a promoter but no LRE and without a promoter or the LRE.  
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Latest revision as of 03:59, 28 October 2010