Team:Cambridge/Bioluminescence/Bacterial Luciferases

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* Design of a biosensor output device that can be combined with various input systems.
* Design of a biosensor output device that can be combined with various input systems.
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Bacterial lux operons encode five enzymes involved in the light-generating pathway. In nature, the lux genes appear to be repressed by the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background nucleoid protein, H-NS], and occur under [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background quorum sensing control]. We wished to relief repression by H-NS to achieve brighter light outputs. We furthermore removed quorum sensing control to facilitate use of the part in biosensors under different input systems.  
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Bacterial lux operons encode five enzymes involved in the light-generating pathway. In nature, the lux genes appear to be repressed by the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background nucleoid protein, H-NS], and occur under [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background quorum sensing control]. We wished to relieve repression by H-NS to achieve brighter light outputs. We furthermore removed quorum sensing control to facilitate use of the part in biosensors under different regulatory inputs.  
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Latest revision as of 03:00, 28 October 2010