Team:Cambridge/Bioluminescence/Bacterial Luciferases

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==Our work==
==Our work==
{{:Team:Cambridge/Templates/RightImage|image=Phosphoreum_bright.jpg|caption=Our workspace illuminated by'' Vibrio phosphoreum'', a bacterium we investigated}}
{{:Team:Cambridge/Templates/RightImage|image=Phosphoreum_bright.jpg|caption=Our workspace illuminated by'' Vibrio phosphoreum'', a bacterium we investigated}}
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To complement 'Project Firefly', we wanted to use lux operons for the following two purposes:
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To complement 'Project Firefly', we intended to use the lux operon from ''Vibrio fischeri'' for the following three purposes:
* Emission of blue light to complete our spectrum of emission wavelengths.
* Emission of blue light to complete our spectrum of emission wavelengths.
* Substrate production within E. coli, avoiding the need for addition of external substrates, such as luciferin.
* Substrate production within E. coli, avoiding the need for addition of external substrates, such as luciferin.
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* Design of a biosensor output device that can be combined with various input systems.
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Bacterial lux operons encode five enzymes involved in the light-generating pathway. In nature, the lux genes appear to be repressed by the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background nucleoid protein, H-NS], and occur under [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background quorum sensing control]. We wished to relieve repression by H-NS to achieve brighter light outputs. We furthermore removed quorum sensing control to facilitate use of the part in biosensors under different regulatory inputs.
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Bacterial lux operons encode five enzymes involved in the light-generating pathway.
 
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*<i>luxA</i> and <i>luxB</i> form the luciferase of the system, which causes the emission of light when acting on the substrate tetradecanal.
 
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*<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates.
 
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In nature, the lux genes appear to be repressed by the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background nucleoid protein, H-NS], and occur under [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background quorum sensing control].
 
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Latest revision as of 03:00, 28 October 2010