Team:Cambridge/Bioluminescence/Bacterial Luciferases

From 2010.igem.org

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*<i>luxA</i> and <i>luxB</i> form the luciferase of the system, which causes the emission of light when acting on the substrate tetradecanal.  
*<i>luxA</i> and <i>luxB</i> form the luciferase of the system, which causes the emission of light when acting on the substrate tetradecanal.  
*<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates.
*<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates.
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In nature, the lux genes appear to be repressed by the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background nucleoid protein, H-NS], and occur under [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background quorum sensing control]. We wished to remove the repression by H-NS and the control through quorum sensing to achieve brighter light outputs and to facilitate use as a biosensor under different input systems, respectively
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In nature, the lux genes appear to be repressed by the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background nucleoid protein, H-NS], and occur under [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background quorum sensing control]. We wished to relief repression by H-NS to achieve brighter light outputs. We furthermore removed control through quorum sensing to facilitate use of the part as a biosensor under different input systems.
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Revision as of 22:37, 27 October 2010