Team:Cambridge/Bioluminescence/Bacterial Luciferases

From 2010.igem.org

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* Substrate production within E. coli, avoiding the need for addition of external substrates, such as luciferin.
* Substrate production within E. coli, avoiding the need for addition of external substrates, such as luciferin.
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Lux operons are found in the genomes of a range of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background natural bioluminescent bacteria]. Such [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background lux systems] contain five genes involved in the generation of light. <i>luxA</i> and <i>luxB</i> form the luciferase of the system, they emit light using the substrate <b>tetradecanal</b>. <i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates.  
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Lux operons are found in the genomes of a range of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background natural bioluminescent bacteria]. Five enzymes are encoded by these light-producing [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background Lux systems].
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*<i>luxA</i> and <i>luxB</i> form the luciferase of the system, which causes the emission of light when acting on the substrate <b>tetradecanal</b>.  
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*<i>luxC</i>, <i>luxD</i> and <i>luxE</i> are involved in the biosynthesis of tetradecanal from readily available substrates.
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In nature, the lux genes appear to be repressed by the [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background nucleoid protein, H-NS], and occur under [https://2010.igem.org/Team:Cambridge/Bioluminescence/Background quorum sensing control].  
==Our work==
==Our work==

Revision as of 18:16, 23 October 2010