Team:Cambridge/Bioluminescence/Bacterial Codon optimisation

From 2010.igem.org

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Ideally we would have submitted a codon-optimised version of the entire Lux operon. Unfortunately Mr Gene, the company we employed for synthesis, still had not completed the order two months after placing, and at the point of the wiki-freeze we still have not received the optimised versions of LuxA and B. In order to complete our aim of creating a PoPs->light device that can be placed under any promoter, we combined the optimised Vibrio LuxCDEG genes with the Edinburgh 2009 Xenorhabdus LuxAB. The assembly was achieved using the [https://2010.igem.org/Team:Cambridge/Gibson/Introduction Gibson method]. Since we only received LuxCD and LuxEG from Mr Gene two weeks before the documentation deadline, we could not properly characterise these parts. For a complete list of the BioBricks we submitted see the [https://2010.igem.org/Team:Cambridge/BioBricks BioBricks section].
Ideally we would have submitted a codon-optimised version of the entire Lux operon. Unfortunately Mr Gene, the company we employed for synthesis, still had not completed the order two months after placing, and at the point of the wiki-freeze we still have not received the optimised versions of LuxA and B. In order to complete our aim of creating a PoPs->light device that can be placed under any promoter, we combined the optimised Vibrio LuxCDEG genes with the Edinburgh 2009 Xenorhabdus LuxAB. The assembly was achieved using the [https://2010.igem.org/Team:Cambridge/Gibson/Introduction Gibson method]. Since we only received LuxCD and LuxEG from Mr Gene two weeks before the documentation deadline, we could not properly characterise these parts. For a complete list of the BioBricks we submitted see the [https://2010.igem.org/Team:Cambridge/BioBricks BioBricks section].
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==Codon optimised bacterial luminescence parts:==
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Codon optimised bacterial luminescence parts:
[http://partsregistry.org/Part:BBa_K325902 luxCD (BBa_K325902)] derived from V. fischeri, but optimised for E.coli
[http://partsregistry.org/Part:BBa_K325902 luxCD (BBa_K325902)] derived from V. fischeri, but optimised for E.coli

Revision as of 22:11, 24 October 2010