Team:Cambridge/Bioluminescence

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(Fireflies in nature)
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Japanese fireflies of the species Luciola cruciata are notable for synchronising their flash patterns when in large groups, thereby making the trees on which they sit pulsate with green light. This phenomenon has been reported to be exploited by fisherman as a means of locating the shore. We wanted to add to the pre-existing luciferase in the Registry by
Japanese fireflies of the species Luciola cruciata are notable for synchronising their flash patterns when in large groups, thereby making the trees on which they sit pulsate with green light. This phenomenon has been reported to be exploited by fisherman as a means of locating the shore. We wanted to add to the pre-existing luciferase in the Registry by
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*Single amino acid mutagenesis to create BioBricks capable of emitting light of a range of different colours.
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*Site-directed mutagenesis to create BioBricks capable of emitting light of a range of different colours.
Luciola cruciata was specifically chosen for this purpose on the basis of a publication by [http://www.ncbi.nlm.nih.gov/pubmed/1946326 Kajiyama and Nakano] in 1991 that described a suite of single amino acid changes which significantly changed the wavelength of peak emission.  
Luciola cruciata was specifically chosen for this purpose on the basis of a publication by [http://www.ncbi.nlm.nih.gov/pubmed/1946326 Kajiyama and Nakano] in 1991 that described a suite of single amino acid changes which significantly changed the wavelength of peak emission.  
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Revision as of 15:20, 26 October 2010