Team:Cambridge/BioBricks

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{{:Team:Cambridge/Templates/Tablerow|3d=902|title=V. fischeri luxCD|description=This is a translational unit designed to produce <i>V. fischeri</i> luxC and luxD genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. Both proteins are part of the  fatty aldehyde synthesis pathway.  luxC is a reductase and luxD is a acytransferase.  LuxE must also be supplied to complete the pathway for fatty aldehyde synthesis.  Such fatty aldehydes are the substrate for the luxAB bacterial luciferase.}}
{{:Team:Cambridge/Templates/Tablerow|3d=902|title=V. fischeri luxCD|description=This is a translational unit designed to produce <i>V. fischeri</i> luxC and luxD genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. Both proteins are part of the  fatty aldehyde synthesis pathway.  luxC is a reductase and luxD is a acytransferase.  LuxE must also be supplied to complete the pathway for fatty aldehyde synthesis.  Such fatty aldehydes are the substrate for the luxAB bacterial luciferase.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=903|title=V. fischeri luxEG|description=This is a translational unit designed to produce <i>V. fischeri</i> luxE and luxG genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. luxE functions as the synthetase creating fatty aldehydes for bioluminescence. LuxG is a flavin reductase.  }}
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{{:Team:Cambridge/Templates/Tablerow|3d=905|title=luxCDEG pLambda AB|description=This part constitutively expresses <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K216008"> the Xenorhabdus luminescens luxAB luciferase</a>.  It also features a translational unit for the substrate generating luxCDEG.  This means that only when supplied with PoPs will it produce light output. }}
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{{:Team:Cambridge/Templates/Tablerow|3d=906|title=pBAD luxCDEG pLambda AB|description=This is the same as the part above but was built for testing induction by adding the pBAD promoter, making light inducible by addition of L-arabinose. }}
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Revision as of 15:01, 24 October 2010