Team:Caltech/Week 5
From 2010.igem.org
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* Transformation plates: L15 (light-lysis construct): failed; L4: >1000 colonies (will reculture to verify antibiotic resistance); L8: 2 colonies (will also reculture to verify that colonies aren't contamination) | * Transformation plates: L15 (light-lysis construct): failed; L4: >1000 colonies (will reculture to verify antibiotic resistance); L8: 2 colonies (will also reculture to verify that colonies aren't contamination) | ||
* Will also start cultures of K124017, as all digests of this brick appear incorrect. We want to try again with fresh DNA. | * Will also start cultures of K124017, as all digests of this brick appear incorrect. We want to try again with fresh DNA. | ||
+ | * Live-dead assay results: All plates from the start point in time contained films of cells. The plates created an hour later from glycerol solutions kept at 4˚C showed little change in the amount of cells grown. Plates created from the room temperature solutions, showed either large colonies and/or less developed films. | ||
* Transformed 5uL ligation product for ligations L4-L14 (again). | * Transformed 5uL ligation product for ligations L4-L14 (again). | ||
* Hydrolyzed soybean oil | * Hydrolyzed soybean oil | ||
}} | }} |
Revision as of 23:13, 19 July 2010
People
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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 7/12
Tuesday 7/13
Wednesday 7/14
Thursday 7/15
Friday 7/16
Weekend 7/17-18
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