Team:Caltech/Week 4
From 2010.igem.org
(Difference between revisions)
Line 36: | Line 36: | ||
==Friday 7/9== | ==Friday 7/9== | ||
+ | * Digested bricks according to NEB BioBrick assembly kit protocol. | ||
+ | ** [http://partsregistry.org/Part:BBa_R0077 R0077] (both upstream and downstream) | ||
+ | ** [http://partsregistry.org/Part:BBa_C0077 C0077] (upstream) | ||
+ | ** [http://partsregistry.org/Part:BBa_C0076 C0076] (upstream) | ||
+ | ** [http://partsregistry.org/Part:BBa_M30109 M30109] (downstream) | ||
+ | * Began ligation reactions | ||
+ | ** [http://partsregistry.org/Part:R0082 R0082]/[http://partsregistry.org/Part:BBa_R0077 R0077]/[http://partsregistry.org/Part:pSB1T3 pSB1T3] | ||
+ | ** [http://partsregistry.org/Part:C0077 C0077]/[http://partsregistry.org/Part:B0015 B0015 (previously digested)]/[http://partsregistry.org/Part:pSB1T3 pSB1T3] | ||
+ | ** [http://partsregistry.org/Part:C0076 C0076]/[http://partsregistry.org/Part:B0015 B0015 (previously digested)]/[http://partsregistry.org/Part:pSB1T3 pSB1T3] | ||
+ | * Transformed ligation products into DH5alpha cells. | ||
+ | * Created liquid cultures | ||
+ | ** ligation 1 product - [http://partsregistry.org/Part:C0076 C0076]/[http://partsregistry.org/Part:B0015 B0015] (previously digested)/[http://partsregistry.org/Part:pSB1T3 pSB1T3] | ||
+ | ** ligation 2 product - [http://partsregistry.org/Part:C0077 C0077]/[http://partsregistry.org/Part:B0015 B0015] (previously digested)/[http://partsregistry.org/Part:pSB1T3 pSB1T3] | ||
+ | ** ligation 3 product - [http://partsregistry.org/Part:R0082 R0082]/[http://partsregistry.org/Part:BBa_R0077 R0077]/[http://partsregistry.org/Part:pSB1T3 pSB1T3] | ||
+ | ** [http://partsregistry.org/Part:B0015 B0015] | ||
+ | ** [http://partsregistry.org/Part:K124017 K124017] | ||
==Weekend 7/10-11== | ==Weekend 7/10-11== | ||
+ | * Made minipreps of [http://partsregistry.org/Part:B0015 B0015] and [http://partsregistry.org/Part:K124017 K124017] | ||
+ | * Transformation results: Transformations failed. | ||
+ | ** There could be a problem with the antibiotic used or there could also be an issue with the ligations. | ||
+ | |||
| | ||
}} | }} |
Revision as of 19:51, 12 July 2010
People
|
Back to Notebook
Monday 7/5Note: Today was another Institute Holiday. Tuesday 7/6
Wednesday 7/7
Thursday 7/8
Friday 7/9
Weekend 7/10-11
|