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Team:Caltech/Week 3 - 2010.igem.org
 

Team:Caltech/Week 3

From 2010.igem.org

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==Monday 6/28==
==Monday 6/28==
* Tested the success of the construct we made using digest and gel electrophoresis.
* Tested the success of the construct we made using digest and gel electrophoresis.
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** Digested ligation product from 6/26 with EcoRI and PstI.
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** Digested ligation product from [https://2010.igem.org/Team:Caltech/Week_2#Saturday_6.2F26 6/26] with EcoRI and PstI.
** Ran digests on gel along with 1kb and 100kb molecular ladders.
** Ran digests on gel along with 1kb and 100kb molecular ladders.
* Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
* Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.

Revision as of 00:25, 29 June 2010


iGEM 2010



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Contents

Monday 6/28

  • Tested the success of the construct we made using digest and gel electrophoresis.
    • Digested ligation product from 6/26 with EcoRI and PstI.
    • Ran digests on gel along with 1kb and 100kb molecular ladders.
  • Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
  • Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells

Tuesday 6/29

Wednesday 6/30

Thursday 7/1

Friday 7/2

Weekend 7/3-4

 
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