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Team:Caltech/Week 15 - 2010.igem.org
 

Team:Caltech/Week 15

From 2010.igem.org

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(New page: {{Caltech_iGEM_10| Content= {{:Team:Caltech/WeekTemplate}} ==Monday 9/20== ==Tuesday 9/21== ==Wednesday 9/22== ==Thursday 9/23== ==Friday 9/24==   }})
 
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==Monday 9/20==
==Monday 9/20==
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*Transformation of completed sigma32 (HtpR) RFC10 BioBrick construction into electrocompetent DH5 alpha cells
==Tuesday 9/21==
==Tuesday 9/21==
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*Miniprep of DNA from Monday's transformed cells
==Wednesday 9/22==
==Wednesday 9/22==
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*Verify that miniprepped plasmid DNA is of sufficiently high concentration to send out for sequencing
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*Send out plasmid DNA for sequencing
==Thursday 9/23==
==Thursday 9/23==
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==Friday 9/24==
==Friday 9/24==
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 +
*Sequencing apparently showed that L36 and L37 did not ligate properly at the second stage of PCR ligation.
 
 
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Latest revision as of 18:16, 17 October 2010


iGEM 2010



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Monday 9/20

  • Transformation of completed sigma32 (HtpR) RFC10 BioBrick construction into electrocompetent DH5 alpha cells

Tuesday 9/21

  • Miniprep of DNA from Monday's transformed cells

Wednesday 9/22

  • Verify that miniprepped plasmid DNA is of sufficiently high concentration to send out for sequencing
  • Send out plasmid DNA for sequencing

Thursday 9/23

Friday 9/24

  • Sequencing apparently showed that L36 and L37 did not ligate properly at the second stage of PCR ligation.


 
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