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Team:Caltech/Protocols
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(New page: * Making V1012 competent * Making stock solutions * Freezer (glycerol) stocks * Plating antibiotics * Sequencing primers * Making synthase casette ** Sources of sequences ** Optimizing & c...)
Newer edit →
(New page: * Making V1012 competent * Making stock solutions * Freezer (glycerol) stocks * Plating antibiotics * Sequencing primers * Making synthase casette ** Sources of sequences ** Optimizing & c...)
Newer edit →
Revision as of 00:04, 2 July 2010
- Making V1012 competent
- Making stock solutions
- Freezer (glycerol) stocks
- Plating antibiotics
- Sequencing primers
- Making synthase casette
- Sources of sequences
- Optimizing & checking genes
- Chemical/Crosslinking reactions
- Digesting substrate (hydrolysis) + epoxidation (MCPBA)
- crosslinking (AIBN3) + UV initiation
- Inducing lysis via 632nm light initiation
- Making cell/glycerol/agar mixture
- Applying this to plates
