Team:Caltech

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Abstract

Our project aims to use dual-wavelength light transduction to produce arbitrary 3D shapes of polyhydroxybutyrate (PHB) plastic from soybean oil. Using a radical crosslinking reagent, we will crosslink PHB oligomers in cell lysate, released upon a light-induced lysis gene network. We hope to apply this capability to three dimensional printing, offering a cheap alternative to current rapid-prototyping technologies. We also plan to apply special consideration to the ramifications of possible commercial enterprises developed in iGEM competitions with open source materials, such as BioBricks.

Specific goals include:

  • Maximize the speed of the printing process
  • Minimize the resources and know-how required to print
  • Maximize the resolution of our printer
  • Maximize the hardness of the plastic produced
  • Minimize the smell of bacteria in the final product
  • Create colored plastic using bacterial pigments
  • Research crosslinking other compounds to create hydrogels instead of plastic














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