Team:Calgary/Project/Achievements

From 2010.igem.org

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<h2 style="color:#0066CC">Overview of things completed</h2>
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<img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/19-1.png" border="0" alt="Photobucket"></img></td>
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<td>malE, malE31, malEΔSS, ibpAB Fusion Promoter, cpxP Promoter</td>
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cpxR reporter, degP reporter, ibpAB reporter, malE generator, malE31 generator, pBad/araC reporters, malE31 generator with cpxR reporter circuit</td>
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<td><img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/20-1.png" border="0" alt="Photobucket"></img></td>
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<img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/21-1-1.png" border="0" alt="Photobucket"></img>
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<td>malE, malE31, ibpAB reporter, cpxR reporter, degP reporter</td>
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<h2 style="color:#0066CC">Bronze Medal Requirements</h2>
<h2 style="color:#0066CC">Bronze Medal Requirements</h2>
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<td>INSERT CHECK MARK HERE</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
<td>Register the team</td>
<td>Register the team</td>
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<td>INSERT CHECK MARK HERE</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
<td>Successfully complete and submit a project summary form</td>
<td>Successfully complete and submit a project summary form</td>
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<td>INSERT CHECK MARK HERE</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
<td>Create a Wiki which includes all the details of the project</td>
<td>Create a Wiki which includes all the details of the project</td>
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<td>INSERT CHECK MARK HERE</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
<td>Present a presentation and a poster at the iGEM Jamboree</td>
<td>Present a presentation and a poster at the iGEM Jamboree</td>
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<td>INSERT CHECK MARK HERE<br/>For more information, please see our Parts page <a href="http://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /><br/>Please see our <br/>Parts page <a href="http://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
<td>Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts</td>
<td>Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts</td>
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<td>INSERT CHECK MARK HERE<br/>For more information, please see our Parts page <a href="http://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /><br/>Please see our <br/>Parts page <a href="http://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
<td>Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts</td>
<td>Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts</td>
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<td> <a style="height:50px; width:50px;"href="http://s872.photobucket.com/albums/ab287/iGEMCalgary_2010/?action=view&current=Checkmark.png" target="_blank"><img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" border="0" alt="Check Mark"></a> </td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /> </td>
<td><h3>Demonstrate that one of your parts works as expected</h3></td>
<td><h3>Demonstrate that one of your parts works as expected</h3></td>
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<li>Through our characterization data, we showed that several of our parts worked as expected. Click <a href="http://2010.igem.org/Team:Calgary/Parts/Characterization"> here</a> for more information.
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<li>Using various methods of characterization, we confirmed that our CpxR reporter circuit was functional. In adition, our maltose binding protein (MalE) and mutant maltose binding protein (MalE31) were shown to be functional. Click <a href="http://2010.igem.org/Team:Calgary/Parts/Characterization"> here</a> for more information.</li>
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<p>The CpxR reporter was found  to report on  misfolding protein.  We characterized this promoter with varying concentrations of folding and misfolding proteins produced which we assumed is correlated to arabinose concentrations due to the use of AraC promoter.  We also tested this promoter with NlpE, an outer membrane lipoprotein that literature has found activates the Cpx pathway.  Finally, we tested this promoter in varying temperature to produce a heat shock response.</p></li>
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<td>INSERT CHECK MARK HERE</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
<td><h3>Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts</h3></td>
<td><h3>Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts</h3></td>
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<li>Both MalE and MalE31 also worked as expected, malE folding in the periplasm and malE31 showing a misfolding response.  This was indicated  through testing with a reporter from another lab (Raivio) (for more info click here) as well as tetsing with our cpxR reporter click here.</li>
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<li>The CpxR reporter, MalE31, and MalE were shown to be functional through characterization. Click <a href="http://2010.igem.org/Team:Calgary/Parts/Parts"> here</a> for more information.</li>
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<li>We characterized the cpxR reporter, malE31, MalE and IbpAB.  See characterization data here.</li></ul>
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<h2 style="color:#0066CC">Gold Medal Requirements</h2>
<h2 style="color:#0066CC">Gold Medal Requirements</h2>
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<td>INSERT CHECK MARK HERE</td>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
<td><h3>Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry</h3></td>
<td><h3>Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry</h3></td>
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<li>we entered new DNA as well as new sequences for malE and malE31.</li>
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<li>More characterization was given to the Registry of Standard Parts for the cpxR promoter as well as for the degP promoter. Details can be found on our characterization page <a href="http://2010.igem.org/Team:Calgary/Parts/Characterization">here</a>. </li>
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<li>we entered characterization data for the CpxR promoter.</li>
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<td>INSERT CHECK MARK HERE</td>
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<td> <img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
<td><h3>Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system </h3></td>
<td><h3>Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system </h3></td>
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<li>We tested out a part for Lethbridge.  We characterized it for possible misfolding in the cytoplasm.  Results can be found here.</li>
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<li>We tested out a part (nms6) for the University of Lethbridge.  We assayed it for possible misfolding in the cytoplasm.  Results can be found <a href="http://2010.igem.org/Team:Calgary/Parts/Characterization"> here</a>.</li>
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<li>We also attended a regional workshop as well as a mock iGEM event with the University of Alberta as well as the University of Lethbridge.  Here we helped each their out with our projects by practicing our presentations for each other, giving suggestions, future directions as well as troubleshooting</li>
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<li> We attended an Alberta-wide conference where teams from the University of Calgary, the University of Lethbridge, and the University of Alberta did mock presentations, gave each other future directions, and gave possible troubleshooting methods.</li>
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<li>Finally our team filled out surveys for the following teams:</li>
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<li>We filled out surveys for the University of Warsaw and the University of Edinburgh iGEM teams</li>
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<td> <img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation</h3></td>
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<li>This year we chose to approach ethics by making a podscasts covering a few different ethical issues pertaining to synthetic biology. We felt tat this would be a novel way to increase awareness about the field of synthetic biology while having a discussion of important issues. We felt that this would be a much more accessible way for the public to gain a better understanding of what the real fears in synthetic biology is as well as to dispel some of the misleading impressions given by media sources.</li>
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<li>We chose to use current social media in the form of podcasts which covered different ethical issues pertaining to synthetic biology. These are posted <a href="http://2010.igem.org/Team:Calgary/Community/Podcasts">here</a>. In addition to this, an analysis of the social, ethical, and economical factors which our project dealt with was written and is posted <a href="http://2010.igem.org/Team:Calgary/Community/Ethics">here</a></li>
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Latest revision as of 04:00, 28 October 2010

Achievements

Overview of things completed

Photobucket malE, malE31, malEΔSS, ibpAB Fusion Promoter, cpxP Promoter
cpxR reporter, degP reporter, ibpAB reporter, malE generator, malE31 generator, pBad/araC reporters, malE31 generator with cpxR reporter circuit Photobucket
Photobucket malE, malE31, ibpAB reporter, cpxR reporter, degP reporter

Bronze Medal Requirements

Register the team
Successfully complete and submit a project summary form
Create a Wiki which includes all the details of the project
Present a presentation and a poster at the iGEM Jamboree

Please see our
Parts page here.
Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts

Please see our
Parts page here.
Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts

Silver Medal Requirements

Demonstrate that one of your parts works as expected

  • Using various methods of characterization, we confirmed that our CpxR reporter circuit was functional. In adition, our maltose binding protein (MalE) and mutant maltose binding protein (MalE31) were shown to be functional. Click here for more information.
  • Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts

  • The CpxR reporter, MalE31, and MalE were shown to be functional through characterization. Click here for more information.
  • Gold Medal Requirements

    Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry

    • More characterization was given to the Registry of Standard Parts for the cpxR promoter as well as for the degP promoter. Details can be found on our characterization page here.

    Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system

    • We tested out a part (nms6) for the University of Lethbridge. We assayed it for possible misfolding in the cytoplasm. Results can be found here.
    • We attended an Alberta-wide conference where teams from the University of Calgary, the University of Lethbridge, and the University of Alberta did mock presentations, gave each other future directions, and gave possible troubleshooting methods.
    • We filled out surveys for the University of Warsaw and the University of Edinburgh iGEM teams
    • Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation

    • We chose to use current social media in the form of podcasts which covered different ethical issues pertaining to synthetic biology. These are posted here. In addition to this, an analysis of the social, ethical, and economical factors which our project dealt with was written and is posted here