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Team:Calgary/9 June 2010
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| - | Himika | + | Himika : Today I Miniprepped the overnight cultures of B0034 and B0015 which were made yesterday. A colony PCR of the B0034-E1010 construct was done as well in order to verify that the construction worked. |
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| + | Emily: Today I ran my restriction digest of I0500 and I13453 on a gel. The band sizes were not quite what was exepcted. The I13453 was not cut at all as there was only 1 single band in each lane. I repeated this digest overnight and the results will be visualized tomorrow. The I0500 was cut, however Colony 1 showed bands much larger than expected so I will proceed with colony 2. I did a construction digest of I0500 C2 with B0034 and then transformed into competant TOP10 cells and plated for overnight growth. Today I also spent some time cleaning out the freezer and labelling boxes so that we can start to organize our constructs. | ||
Dev, Chris, and Jeremy: Today, they Miniprepped the plasmid backbones pSB1A2, pSB1AC3, pSB1AK3, and pSB1K3 so they could be used in a plasmid transfer. Then, they made constructs to put the parts E0032 and E0040 into the kanamycin plasmids which could then be grown overnight. | Dev, Chris, and Jeremy: Today, they Miniprepped the plasmid backbones pSB1A2, pSB1AC3, pSB1AK3, and pSB1K3 so they could be used in a plasmid transfer. Then, they made constructs to put the parts E0032 and E0040 into the kanamycin plasmids which could then be grown overnight. | ||
Alex and Patrick | Alex and Patrick | ||
Revision as of 21:16, 9 June 2010
Himika : Today I Miniprepped the overnight cultures of B0034 and B0015 which were made yesterday. A colony PCR of the B0034-E1010 construct was done as well in order to verify that the construction worked.
Emily: Today I ran my restriction digest of I0500 and I13453 on a gel. The band sizes were not quite what was exepcted. The I13453 was not cut at all as there was only 1 single band in each lane. I repeated this digest overnight and the results will be visualized tomorrow. The I0500 was cut, however Colony 1 showed bands much larger than expected so I will proceed with colony 2. I did a construction digest of I0500 C2 with B0034 and then transformed into competant TOP10 cells and plated for overnight growth. Today I also spent some time cleaning out the freezer and labelling boxes so that we can start to organize our constructs.
Dev, Chris, and Jeremy: Today, they Miniprepped the plasmid backbones pSB1A2, pSB1AC3, pSB1AK3, and pSB1K3 so they could be used in a plasmid transfer. Then, they made constructs to put the parts E0032 and E0040 into the kanamycin plasmids which could then be grown overnight.
Alex and Patrick
