Team:Calgary/9 July 2010

From 2010.igem.org

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<u>Himika</u>
<u>Himika</u>
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Today I did a restriction digest of parts with Eco/Spe in buffer IIthat I constructed and the plasmid switch I did on July 6th.
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Today I did a restriction digest of parts with Eco/Spe in buffer II that I constructed and the plasmid switch I did on July 6th.
The parts that I digested were:
The parts that I digested were:
*3 colonies of E1010 in AC plasmid  
*3 colonies of E1010 in AC plasmid  
*2 colonies of E1010-B0015 construction done on July 6th
*2 colonies of E1010-B0015 construction done on July 6th
*I also ran  2 colonies of I13507
*I also ran  2 colonies of I13507
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*1 colony of E1010 Kan
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*1 colony of E1010 in its original Kanamycin plasmid
Refer to the image for more details about the restriction digest.
Refer to the image for more details about the restriction digest.
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I also updated the team and Dr. Schryvers about the IbpAB protein and modelling that I did this and last week.
I also updated the team and Dr. Schryvers about the IbpAB protein and modelling that I did this and last week.
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I also designed primer along with Dave for the pRFP circuit.
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I also designed primer along with Dave for the ppRFP circuit.
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Revision as of 20:11, 9 July 2010

restriction digest of parts that Himika ran on 07-09-2010

Friday July 9, 2010


Himika

Today I did a restriction digest of parts with Eco/Spe in buffer II that I constructed and the plasmid switch I did on July 6th. The parts that I digested were:

  • 3 colonies of E1010 in AC plasmid
  • 2 colonies of E1010-B0015 construction done on July 6th
  • I also ran 2 colonies of I13507
  • 1 colony of E1010 in its original Kanamycin plasmid

Refer to the image for more details about the restriction digest.

I also updated the team and Dr. Schryvers about the IbpAB protein and modelling that I did this and last week.

I also designed primer along with Dave for the ppRFP circuit.

No notebook page exists for this date. Sorry!