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Team:Calgary/8 June 2010
From 2010.igem.org
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Alex and Patrick: Patrick and Alex made overnight cultures of E0430 and R0040 as well as helping finish the Miniprep of Emily and Himika's genes. | Alex and Patrick: Patrick and Alex made overnight cultures of E0430 and R0040 as well as helping finish the Miniprep of Emily and Himika's genes. | ||
| - | Emily and Himika: Emily and Himika made overnight cultures from B0034 (the RBS that we all need) as well as finishing Emily's colony PCR and MiniPrep for I0500 and I3453. | + | Emily and Himika: Emily and Himika made overnight cultures from B0034 (the RBS that we all need) and B0015 (the double terminator for the end of all our circuits) as well as finishing Emily's colony PCR and MiniPrep for I0500 and I3453. |
Revision as of 20:07, 9 June 2010
Today, we spent most of the day making overnight cultures from the transformed plates that were made yesterday. As a group, we began getting fundraising underway by finding contacts, dividng them among each person and setting a tentative goal of $20 000 to fundraise from corporate sponsors. We also organized how the money given from Alberta Innovates would be used
Chris, Jeremy, and Dev: Our group spent today making overnight cultures so plasmid transfers could be done tomorrow. We made the cultures for plasmids pSB1A3, pSB1K2, pSB1AC3 and pSB1AK3 as well as restreaking the colonies that were used to innoculate the overnight cultures. Jeremy also transformed I732005 (lacZ gene for B-galactosidase) into competent cells so they could be used.
Alex and Patrick: Patrick and Alex made overnight cultures of E0430 and R0040 as well as helping finish the Miniprep of Emily and Himika's genes.
Emily and Himika: Emily and Himika made overnight cultures from B0034 (the RBS that we all need) and B0015 (the double terminator for the end of all our circuits) as well as finishing Emily's colony PCR and MiniPrep for I0500 and I3453.
