Sunday August 8, 2010

Dev

In the lab on a Sunday again! Made overnight cultures of the 8 colonies that showed correct sized bands from the colony PCR performed on Friday. These cultures will be plasmid prepped first thing tomorrow morning.

Emily

Late Sunday nights in the lab are the best! Tonight I finished the construction to try to get PCR [urified (linear) malE and malE31 into the psB1AC3 and psB1AK3 vectors. I transformed these into E. Coliu TOP10 cells and then plated on C and K plates and left for overnight growth. I also made overnight cultures of my I0500-I13504 and I0500-I13507 constructs. These will be miniprepped tomorrow and then verification digests will be performed before we perform the Arabinose Induction trials again. I also set up two more gradient PCR reactions of malESSdel and malE31SSdel. We are hoping to get enough amplification from these trhat we can proceed to a PCR purification in order to try to construct these (hopefully) biobricked genes into biobrick construction plasmids. Tonight Chris and I also made ovenright cultures of TOP10 cells in order to strat making more TOP10 Competant cells tomorrow.