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Team:Calgary/7 September 2010
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| + | Today I PCR Purified malE and malE31. I then digested these as well as the psB1AK3 vector with a combination of restriction enzymes to try to get it into an AK BBK construction vector. I ligated these and transformed them into TOP10 competant cells and plated on K plates, leaving for overnight growth. Today we also all worked on the presentation that we will be giving at the 3rd annual aGEM Jamboree this coming weekend in Edmonton. | ||
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Revision as of 17:45, 7 September 2010
Tuesday September 7, 2010
Emily
Today I PCR Purified malE and malE31. I then digested these as well as the psB1AK3 vector with a combination of restriction enzymes to try to get it into an AK BBK construction vector. I ligated these and transformed them into TOP10 competant cells and plated on K plates, leaving for overnight growth. Today we also all worked on the presentation that we will be giving at the 3rd annual aGEM Jamboree this coming weekend in Edmonton.
