Team:Calgary/5 August 2010

From 2010.igem.org

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{{CalgaryNotebookTemplate|
{{CalgaryNotebookTemplate|
'''Thursday August 5, 2010'''
'''Thursday August 5, 2010'''
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<u>Dev</u>
<u>Dev</u>
OBTAINED COLONIES! Six colonies from each of the four plates were chosen to be tested for the correct construct (K135000+I13504 or K135000+I13507) by means of a colony PCR.
OBTAINED COLONIES! Six colonies from each of the four plates were chosen to be tested for the correct construct (K135000+I13504 or K135000+I13507) by means of a colony PCR.
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<u> Raida </u>
<u> Raida </u>
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Today I ran a PCR of the following templates: plasmids containing MalE and MalE 31 with the signal sequence deleted. The primers are specific to signal sequence deleted MalE and MalE 31 genes.  
Today I ran a PCR of the following templates: plasmids containing MalE and MalE 31 with the signal sequence deleted. The primers are specific to signal sequence deleted MalE and MalE 31 genes.  
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<u>Chris</u>
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Today, I spent the day contacting sponsors and preparing the sponsorship letters that would be used to contact the different faculties. I found colonies of the pSB1AC3 and pSB1AK3 from the registry and made overnight cultures to be Miniprepped tomorrow. Finally, I began the digest of the CpxP promoter in preparation of inserting it into a plasmid.
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Revision as of 23:33, 5 August 2010

Thursday August 5, 2010


Dev

OBTAINED COLONIES! Six colonies from each of the four plates were chosen to be tested for the correct construct (K135000+I13504 or K135000+I13507) by means of a colony PCR.


Raida

Today I ran a PCR of the following templates: plasmids containing MalE and MalE 31 with the signal sequence deleted. The primers are specific to signal sequence deleted MalE and MalE 31 genes.


Chris

Today, I spent the day contacting sponsors and preparing the sponsorship letters that would be used to contact the different faculties. I found colonies of the pSB1AC3 and pSB1AK3 from the registry and made overnight cultures to be Miniprepped tomorrow. Finally, I began the digest of the CpxP promoter in preparation of inserting it into a plasmid.

No notebook page exists for this date. Sorry!