Team:Calgary/30 September 2010

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[[Image:09.28.2010-I0500-B0034-MALE rd.jpg|thumb|right|400px|Restriction digest of I0500-B0034-MalE colonies 1-12, Lane 1 is control]]
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<u>Himika</u>
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I did another restriction digest of I0500-B0034-MalE since the restriction digest last night failed. This time I tried with EcoRI and SpeI. The restriction digest worked and Colony 10 will be sent down for sequencing tomorrow. The plates from last night  did not grow either. So I retried the construction by retrying the restriction digest. I left the digest overnight and will plate tomorrow.
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Latest revision as of 04:12, 17 October 2010

Thursday September 30, 2010

Restriction digest of I0500-B0034-MalE colonies 1-12, Lane 1 is control

Himika

I did another restriction digest of I0500-B0034-MalE since the restriction digest last night failed. This time I tried with EcoRI and SpeI. The restriction digest worked and Colony 10 will be sent down for sequencing tomorrow. The plates from last night did not grow either. So I retried the construction by retrying the restriction digest. I left the digest overnight and will plate tomorrow.