Team:Calgary/30 August 2010

From 2010.igem.org

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{{CalgaryNotebookTemplate|
{{CalgaryNotebookTemplate|
Monday August 30, 2010|
Monday August 30, 2010|
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[[Image:Omen.jpg|400px|thumb|Uh-oh...is this an omen for things to come?]]
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<u>Raida</u>
<u>Raida</u>
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Trying very hard to get a strand of amino acids (i.e. a very long cylinder object) to twist and fold into a "protein" (i.e. a very long cylinder object knotted together). Skeleton and inverse kinematic animation is proving much more difficult than it first appears to be.
Trying very hard to get a strand of amino acids (i.e. a very long cylinder object) to twist and fold into a "protein" (i.e. a very long cylinder object knotted together). Skeleton and inverse kinematic animation is proving much more difficult than it first appears to be.
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<u>Chris</u>
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Today, I did plasmid preparations of the construction of (I0500-I13507 which will be given to Dev to verify. As well, I made ampicillin containing plates and made overnight cultures of the CpxP promoter and the ibpAB-fsxA-T7 fusion promoter inserted into the AK and AC plasmid. I continued my reading into DegP and Cpx which the paper was due last week...I will get that to you as soon as possible, Emily.
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Revision as of 00:01, 31 August 2010

Monday August 30, 2010

Uh-oh...is this an omen for things to come?


Raida

Today, I set up a PCR Reaction in order to biobrick MalE and MalE31 without the signalling sequence. I set up 8x with the MalE-BBKdelss-F and the MalE-R primers. The negative control is a MalE plasmid. Another set of reaction was set, which is with the MalE-BBk-F and the MalE-R and the positive control was MalE plasmid.

I have also completed my draft with suggestion made from Himika and Paul on my introduction and also which section should have the paragraphs that I had written. I also wrote for the Legal portion of the paper.


Jeremy

Today is my first real day of wetlab stuff since my time off. I redid the pRFP biobrick and will run the gel tomorrow. I also did a transformation of MalE, MalE31 and NlpE into the Ravio competent cells. I also made more agar and broth.

Non-wetlab stuff that I did included fixing areas of the wiki, writing up the transcription/translation portion of the circuit and doing the Calgary, University of Calgary and O'brien sections of the wiki.


Patrick

Trying very hard to get a strand of amino acids (i.e. a very long cylinder object) to twist and fold into a "protein" (i.e. a very long cylinder object knotted together). Skeleton and inverse kinematic animation is proving much more difficult than it first appears to be.


Chris

Today, I did plasmid preparations of the construction of (I0500-I13507 which will be given to Dev to verify. As well, I made ampicillin containing plates and made overnight cultures of the CpxP promoter and the ibpAB-fsxA-T7 fusion promoter inserted into the AK and AC plasmid. I continued my reading into DegP and Cpx which the paper was due last week...I will get that to you as soon as possible, Emily.