Team:Calgary/23 October 2010

From 2010.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 2: Line 2:
Saturday October 23, 2010|
Saturday October 23, 2010|
-
<u>Emily<u>
+
<u>Emily</u>
Today I set up a massive colony PCR with the BBK-CP primers in order to veirfy that we were able to get all of our parts into the psB1C3 vector.  Today I also made more LB broth.  I also did transformations of a few other parts into the psB1C3 vector as well as the construction of I0500-B0034 with the Lethbridge part.
Today I set up a massive colony PCR with the BBK-CP primers in order to veirfy that we were able to get all of our parts into the psB1C3 vector.  Today I also made more LB broth.  I also did transformations of a few other parts into the psB1C3 vector as well as the construction of I0500-B0034 with the Lethbridge part.
 +
 +
<u>Himika</u>
 +
 +
Today I set subcultured the DegP cells containing MalE and MalE31 into 20 ml broth and allowed it to grow for 6 hours. I induced these cells with 15 different arabinose concentrations. This was left shaking overnight and I will read these cells tomorrow.
}}
}}

Latest revision as of 01:03, 27 October 2010

Saturday October 23, 2010

Emily

Today I set up a massive colony PCR with the BBK-CP primers in order to veirfy that we were able to get all of our parts into the psB1C3 vector. Today I also made more LB broth. I also did transformations of a few other parts into the psB1C3 vector as well as the construction of I0500-B0034 with the Lethbridge part.


Himika

Today I set subcultured the DegP cells containing MalE and MalE31 into 20 ml broth and allowed it to grow for 6 hours. I induced these cells with 15 different arabinose concentrations. This was left shaking overnight and I will read these cells tomorrow.