Team:Calgary/21 June 2010

From 2010.igem.org

Monday June 21, 2010

Emily: Today I did another verification digest of the arabionose promoter (I0500). This time, I tried digesting with some none Biobrick restriction enzymes that have cut sites in the middle of the gene. Hopfeully, if this gives us bands of expected sizes, we can be sure that the arabinose promoter is what we think that it is. Today I also ran a gel of the verifictaion restriction digest that I perfomred on Friday. See the photo of the gel below.

Dev, Jeremy, and Chris: Today, Jeremy did gel electrophoresis of the restriction digest of his previous construct of B0015-R0040 using Biobrick restriction enzymes to test if the parts were correctly placed together. If the bands give show as expected, it is likely that the construct is what we expected it to be. The results of the gel electophoresis were inconsistent and as such, must be redone tomorrow. Chris did the construction of E0032-B0015 which is the end of the mRNA verification circuit. This was done by restriction digest, ligation, and transformation of the DNA into Top10 competent cells. Dev made overnight cultures of Registry parts J13002, J23032 and E0032 (kan plasmid transferred) so they could be Miniprepped tomorrow and ready for use.