Team:Calgary/21 July 2010

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Revision as of 22:00, 21 July 2010 by Raidakhwaja (Talk | contribs)

Wednesday July 21, 2010

Raida:

Objective: PCR out native MAlE/ MalE 31 protein out of E.coli Purpose: Testing Mal E/ Mal E31 primers to get 1188 bp long gene of interest. Primers that are used are gene specific and anneals right on the start of the Mal E gene.

Tube Labels

  • 1- p1H - C1
  • 2- p1H - C1
  • 3- p1H - C2
  • 4- p1H - C2
  • 5- p31H - C1
  • 6- p31H - C2
  • 7- p1ΔSS - C1
  • 8- Master Mix

PCR Conditions Cycle 1: 94°C 2:00 min Cycle 2:

  • Stage 1:94°C for 1:00 min
  • Stage 2:52°C for 0:45 min
  • Stage 3:72°C for 1:15 min
  • Cycle 3: 72°C for 10:00 min

Holding Temp: 4°C


No notebook page exists for this date. Sorry!