Team:Calgary/21 July 2010

From 2010.igem.org

(Difference between revisions)
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'''PCR Conditions'''
'''PCR Conditions'''
*Cycle 1: 94°C 2:00 min
*Cycle 1: 94°C 2:00 min
-
*Cycle 2:
+
*Cycle 2 * 36 cycles:
**Stage 1:94°C  for  1:00 min
**Stage 1:94°C  for  1:00 min
**Stage 2:52°C  for  0:45 min
**Stage 2:52°C  for  0:45 min

Revision as of 22:03, 21 July 2010

Wednesday July 21, 2010

Raida:

Objective: PCR out native MalE/ MalE 31 protein out of E.coli Purpose: Testing Mal E/ Mal E31 primers to get 1188 bp long gene of interest. Primers that are used are gene specific and anneals right on the start of the Mal E gene.

Tube Labels containing 1.0 μL of 10 mm DNA

  • 1- p1H - C1
  • 2- p1H - C1
  • 3- p1H - C2
  • 4- p1H - C2
  • 5- p31H - C1
  • 6- p31H - C2
  • 7- p1ΔSS - C1
  • 8- Master Mix

PCR Conditions

  • Cycle 1: 94°C 2:00 min
  • Cycle 2 * 36 cycles:
    • Stage 1:94°C for 1:00 min
    • Stage 2:52°C for 0:45 min
    • Stage 3:72°C for 1:15 min
  • Cycle 3: 72°C for 10:00 min
    • Holding Temp: 4°C


No notebook page exists for this date. Sorry!