Thurday July 15, 2010

RAIDA:

• Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.

PCR conditions: Stage 1: 94 degrees Celcius Stage 2; Step 1: 94 degrees 1 minute Stage 2; Step 2: 55 degrees 45 seconds Stage 2; Step 3: 72 degrees 1 minute 15 seconds Stage 3: 72 degrees Holding Temperature: 4 degrees Celcius

• I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.