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Team:Calgary/15 July 2010
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'''Thurday July 15, 2010''' | '''Thurday July 15, 2010''' | ||
| - | + | ''RAIDA:'' | |
| + | |||
| + | *Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with. | ||
| + | PCR conditions: | ||
| + | Stage 1: 94 degrees Celcius | ||
| + | Stage 2; Step 1: 94 degrees 1 minute | ||
| + | Stage 2; Step 2: 55 degrees 45 seconds | ||
| + | Stage 2; Step 3: 72 degrees 1 minute 15 seconds | ||
| + | Stage 3: 72 degrees | ||
| + | Holding Temperature: 4 degrees Celcius | ||
| + | |||
| + | *I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp. | ||
| + | |||
| + | |||
}} | }} | ||
Revision as of 21:51, 15 July 2010
Thurday July 15, 2010
RAIDA:
- Today I did a Colony PCR of the the I0050 + B0034 Construct with the primers that Emily provided me with.
PCR conditions: Stage 1: 94 degrees Celcius Stage 2; Step 1: 94 degrees 1 minute Stage 2; Step 2: 55 degrees 45 seconds Stage 2; Step 3: 72 degrees 1 minute 15 seconds Stage 3: 72 degrees Holding Temperature: 4 degrees Celcius
- I have also assisted Alex with his Gel electrophoresis of the CpxP colony pcr. We are hoping to see a band around the length of 150 bp.
