Team:Brown/Project/Light pattern/Future

From 2010.igem.org

(Difference between revisions)
(Future Directions)
(Future Directions)
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Applications:
Applications:
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The four-state Light-Pattern Controlled Circuit has many useful applications to manufacturing of end products which require several enzymatic steps. With the output of each state in the circuit as the enzyme of the subsequent step in the manufacturing process, the synthesis of many compounds may be combined to a one-pot biosynthetic process, significantly cutting costs and risk of contamination. Another advantage of applying the light-pattern controlled circuit is in drawing manufacturers towards the greener biosynthetic processes; this will cutting down on toxic waste that would have accumulated in alternative chemical synthetic processes, which right now yields 25-100 kg of waste per kolgram of product in the average pharmaceutical synthesis (Sheldon, 1997)!   
The four-state Light-Pattern Controlled Circuit has many useful applications to manufacturing of end products which require several enzymatic steps. With the output of each state in the circuit as the enzyme of the subsequent step in the manufacturing process, the synthesis of many compounds may be combined to a one-pot biosynthetic process, significantly cutting costs and risk of contamination. Another advantage of applying the light-pattern controlled circuit is in drawing manufacturers towards the greener biosynthetic processes; this will cutting down on toxic waste that would have accumulated in alternative chemical synthetic processes, which right now yields 25-100 kg of waste per kolgram of product in the average pharmaceutical synthesis (Sheldon, 1997)!   
The field of enzyme enfineering is relatively new and has a great deal of potential for growth Already, there are examples of multistep enzyme-catalyzed processes to which our light-pattern controlled circuit can be applied. An example of this is the synthesis of D-xylulose 5-phosphate synthesis developed by Zimmermann which makes it possible to utilize both triose phophate equivalents that are formed from aldolase-catalyzed fructose diphosphate cleavage (Zimmermann, 1999).  
The field of enzyme enfineering is relatively new and has a great deal of potential for growth Already, there are examples of multistep enzyme-catalyzed processes to which our light-pattern controlled circuit can be applied. An example of this is the synthesis of D-xylulose 5-phosphate synthesis developed by Zimmermann which makes it possible to utilize both triose phophate equivalents that are formed from aldolase-catalyzed fructose diphosphate cleavage (Zimmermann, 1999).  
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[[Image:4.gif‎]]
[[Image:4.gif‎]]
A current limitation of one-pot biosynthesis using transgenic bacteria is the need for site-isolation, the physical separation of catalysts from each other (Broadwater, 2005). With our circuit, this roadblock may be partially remediable by temporal isolation. With this comes the possibility of combining current separated biosynthetic processes into a one-pot synthesis. An example of such a possibility may be in the production of the top-selling drug in the world, Atorvastatin (Lipitor), in which many intermediates can be made through biocatalysts such asalcohol dehydrogenase, aldolase, nitrilase, lipase, halohydrin dehalogenase (Patel, 2009), as shown below.
A current limitation of one-pot biosynthesis using transgenic bacteria is the need for site-isolation, the physical separation of catalysts from each other (Broadwater, 2005). With our circuit, this roadblock may be partially remediable by temporal isolation. With this comes the possibility of combining current separated biosynthetic processes into a one-pot synthesis. An example of such a possibility may be in the production of the top-selling drug in the world, Atorvastatin (Lipitor), in which many intermediates can be made through biocatalysts such asalcohol dehydrogenase, aldolase, nitrilase, lipase, halohydrin dehalogenase (Patel, 2009), as shown below.
‎[[Image:App1.jpg‎]]  
‎[[Image:App1.jpg‎]]  
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[[Image:App2.jpg]]
[[Image:App2.jpg]]
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Citations:
Citations:
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J.M. Patel, J. Mol. Catal. B: Enzym. 61 (2009), pp. 123–128.
 
S. J. Broadwater, S. L. Roth, K. E. Price, M. Kobašlija, D. T. McQuade, Org. Biomol. Chem. 2005, 3, 2899–2906;
S. J. Broadwater, S. L. Roth, K. E. Price, M. Kobašlija, D. T. McQuade, Org. Biomol. Chem. 2005, 3, 2899–2906;
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F.T. Zimmermann, A. Schneider, U. Schörken, G.A. Sprenger and W.-D. Fessner, Tetrahedron: Asymmetry 10 (1999), pp. 1643–1646.
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J.M. Patel, J. Mol. Catal. B: Enzym. 61 (2009), pp. 123–128.
R. A. Sheldon, J. Chem. Technol. Biotechnol., 1997, 68, 381–388
R. A. Sheldon, J. Chem. Technol. Biotechnol., 1997, 68, 381–388
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 +
F.T. Zimmermann, A. Schneider, U. Schörken, G.A. Sprenger and W.-D. Fessner, Tetrahedron: Asymmetry 10 (1999), pp. 1643–1646.

Revision as of 20:10, 27 October 2010

Light-Pattern Controlled Circuit

Future Directions

Construction of full circuit, testing, refining and replacing parts, etc!

Applications:


The four-state Light-Pattern Controlled Circuit has many useful applications to manufacturing of end products which require several enzymatic steps. With the output of each state in the circuit as the enzyme of the subsequent step in the manufacturing process, the synthesis of many compounds may be combined to a one-pot biosynthetic process, significantly cutting costs and risk of contamination. Another advantage of applying the light-pattern controlled circuit is in drawing manufacturers towards the greener biosynthetic processes; this will cutting down on toxic waste that would have accumulated in alternative chemical synthetic processes, which right now yields 25-100 kg of waste per kolgram of product in the average pharmaceutical synthesis (Sheldon, 1997)!

The field of enzyme enfineering is relatively new and has a great deal of potential for growth Already, there are examples of multistep enzyme-catalyzed processes to which our light-pattern controlled circuit can be applied. An example of this is the synthesis of D-xylulose 5-phosphate synthesis developed by Zimmermann which makes it possible to utilize both triose phophate equivalents that are formed from aldolase-catalyzed fructose diphosphate cleavage (Zimmermann, 1999).

4.gif

A current limitation of one-pot biosynthesis using transgenic bacteria is the need for site-isolation, the physical separation of catalysts from each other (Broadwater, 2005). With our circuit, this roadblock may be partially remediable by temporal isolation. With this comes the possibility of combining current separated biosynthetic processes into a one-pot synthesis. An example of such a possibility may be in the production of the top-selling drug in the world, Atorvastatin (Lipitor), in which many intermediates can be made through biocatalysts such asalcohol dehydrogenase, aldolase, nitrilase, lipase, halohydrin dehalogenase (Patel, 2009), as shown below. ‎App1.jpg


App2.jpg


Citations:

S. J. Broadwater, S. L. Roth, K. E. Price, M. Kobašlija, D. T. McQuade, Org. Biomol. Chem. 2005, 3, 2899–2906;

J.M. Patel, J. Mol. Catal. B: Enzym. 61 (2009), pp. 123–128.

R. A. Sheldon, J. Chem. Technol. Biotechnol., 1997, 68, 381–388

F.T. Zimmermann, A. Schneider, U. Schörken, G.A. Sprenger and W.-D. Fessner, Tetrahedron: Asymmetry 10 (1999), pp. 1643–1646.