http://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&feed=atom&action=historyTeam:Brown/Project/Light pattern - Revision history2024-03-29T06:55:21ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=176824&oldid=prevTgjohnst: Replacing page with '{{:Team:Brown/templates/header}}
==Light-Pattern Controlled Circuit==
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Light-Pattern Controlled Circuit==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Light-Pattern Controlled Circuit==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">{{:Team:Brown/templates/toc}}</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We <ins class="diffchange diffchange-inline">don</ins>'<ins class="diffchange diffchange-inline">t know how you got here</ins>, <ins class="diffchange diffchange-inline">but you should click below </ins>to <ins class="diffchange diffchange-inline">return </ins>to our <ins class="diffchange diffchange-inline">site:</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Overview===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[:<ins class="diffchange diffchange-inline">Team</ins>:<ins class="diffchange diffchange-inline">Brown</ins>/<ins class="diffchange diffchange-inline">Project</ins>/<ins class="diffchange diffchange-inline">Light_pattern</ins>/<ins class="diffchange diffchange-inline">Overview</ins>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Our project this year attempts to tackle what we see as emerging issue in synthetic biology: the complexity of circuit inputs. [[Image:Manyinput.jpg|thumb|200px|right|A user may have to apply many inputs to achieve a distinct set of outputs]] There is a developing trend among iGEM projects towards intricate systems; the registry of standard parts is growing quickly and the tools at the disposal of a synthetic biologist are increasing rapidly. While many of these systems rely on an autonomous progression of events in the chasis - say, a cell encounters some environmental stimulus which triggers downstream responses - some require precise user control. This is especially true for projects in the manufacturing area, as often a product is achieved following a progression of steps within the cell. Functioning "behind the scenes" to achieve this sort of controllable progression is the genetic circuit, consisting of plasmids loaded with promoter and transcription factor pairs. Through a combination of regulators and activators, these circuits achieve distinct "states," or set of functions carried out by the cell. Whether chemical, heat, or some other environmental change, it is common practice that for each state, a unique input must be applied. With this approach, it is quite clear that an increasingly complicated circuit, with many different promoters, requires an increasingly intricate set of inputs.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We <del class="diffchange diffchange-inline">believe this to be a problem for a few reasons:</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># Accessibility</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#*From an end user perspective, navigation across states could become nightmarish. Especially for a system targeted beyond the lab, perhaps at a non-technical setting, input/control must be accessible and easily attainable.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># Expense</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#*Chemical induction of individual components of a circuit, particularly on a large bioreactor scale as in telescoping synthesis, is expensive! For a full-fledged, industrial bioreactor, an enormous amount of inducer must be introduced. One chemical input alone on this scale can vastly increase the cost of the end product; multiple chemical inputs drives costs extremely high.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># Efficiency</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#*Environmental changes, such as heat or pH, may remove a cell from optimal conditions for protein production. Again, from a manufacturing standpoint, a decrease in yield per reaction increases costs and decreases overal efficiency.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Where does this leave us? With room for improvement, of course! We sought to address the shortcomings detailed above in engineering of a '''four state circuit''' that is '''not controlled by multiple inputs''</del>'<del class="diffchange diffchange-inline">. To avoid complexity in input</del>, <del class="diffchange diffchange-inline">we created a kind of dependence of each logic component of the circuit on the presence or absence of light. Previous iGEM projects on light control of circuits consists of two state toggling, where each change in light swaps a circuit from state 1 </del>to <del class="diffchange diffchange-inline">state 2 and back.[[Image:theOld.jpg|thumb|300px|left|An illustration of previous light toggled circuits, that alternate between two states corresponding </del>to <del class="diffchange diffchange-inline">the state of light]] Rather than returning to state 1 given a light change back to OFF, </del>our <del class="diffchange diffchange-inline">circuit takes advantage of a memory component to effect a unique progression of states.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>[[<del class="diffchange diffchange-inline">Image</del>:<del class="diffchange diffchange-inline">theNew.jpg|thumb|300px|right|As the light alternates from OFF/ON, our circuit progresses to a third and fourth state!]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">What are the ramifications of this project? We believe that our circuit design, where progression across sets of protein production is controlled solely by light, has the potential to drastically reduce costs in biological manufacturing practices. Furthermore, we hope our project provides a foundational advancement in circuit design by emphasizing the importance of '''abstraction'''; specifically, the separation of user input and the precise molecules that control transcription. Read on to learn more about our project!</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Workflow/Methods===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Modeling===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">See our modeling page at: blah blah</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">This is some text blah blah</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Future Direction===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Tgjohnsthttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=176303&oldid=prevEthan richman: /* Light-Pattern Controlled Circuit */2010-10-27T07:47:35Z<p><span class="autocomment">Light-Pattern Controlled Circuit</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Light-Pattern Controlled Circuit==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Light-Pattern Controlled Circuit==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Overview===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Overview===</div></td></tr>
</table>Ethan richmanhttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=176298&oldid=prevEthan richman: /* Light-Pattern Controlled Circuit */2010-10-27T07:47:17Z<p><span class="autocomment">Light-Pattern Controlled Circuit</span></p>
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</table>Ethan richmanhttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=175956&oldid=prevEthan richman: /* Abstract */2010-10-27T07:23:35Z<p><span class="autocomment">Abstract</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Abstract===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, '''we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light.''' With this production method, '''we can link multiple synthesis steps to a single, clean and rapidly scalable input.'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Overview===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Overview===</div></td></tr>
</table>Ethan richmanhttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=175219&oldid=prevEthan richman: /* Overview */2010-10-27T06:28:13Z<p><span class="autocomment">Overview</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image:theNew.jpg|thumb|300px|right|As the light alternates from OFF/ON, our circuit progresses to a third and fourth state!]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image:theNew.jpg|thumb|300px|right|As the light alternates from OFF/ON, our circuit progresses to a third and fourth state!]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>What are the ramifications of this project? We believe that our circuit design, where progression across sets of protein production is controlled solely by light, has the potential to drastically reduce costs in biological manufacturing practices. Furthermore, we hope our project provides a foundational advancement in circuit design by emphasizing the importance of '''abstraction'''<del class="diffchange diffchange-inline">. We think </del>the</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>What are the ramifications of this project? We believe that our circuit design, where progression across sets of protein production is controlled solely by light, has the potential to drastically reduce costs in biological manufacturing practices. Furthermore, we hope our project provides a foundational advancement in circuit design by emphasizing the importance of '''abstraction'''<ins class="diffchange diffchange-inline">; specifically, </ins>the <ins class="diffchange diffchange-inline">separation of user input and the precise molecules that control transcription. Read on to learn more about our project!</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Workflow/Methods===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Workflow/Methods===</div></td></tr>
</table>Ethan richmanhttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=175107&oldid=prevEthan richman: /* Overview */2010-10-27T06:21:14Z<p><span class="autocomment">Overview</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Where does this leave us? With room for improvement, of course! We sought to address the shortcomings detailed above in engineering of a '''four state circuit''' that is '''not controlled by multiple inputs'''. To avoid complexity in input, we created a kind of dependence of each logic component of the circuit on the presence or absence of light. Previous iGEM projects on light control of circuits consists of two state toggling, where each change in light swaps a circuit from state 1 to state 2 and back.[[Image:theOld.jpg|thumb|300px|left|An illustration of previous light toggled circuits, that alternate between two states corresponding to the state of light]] Rather than returning to state 1 given a light change back to OFF, our circuit takes advantage of a memory component to effect a unique progression of states.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Where does this leave us? With room for improvement, of course! We sought to address the shortcomings detailed above in engineering of a '''four state circuit''' that is '''not controlled by multiple inputs'''. To avoid complexity in input, we created a kind of dependence of each logic component of the circuit on the presence or absence of light. Previous iGEM projects on light control of circuits consists of two state toggling, where each change in light swaps a circuit from state 1 to state 2 and back.[[Image:theOld.jpg|thumb|300px|left|An illustration of previous light toggled circuits, that alternate between two states corresponding to the state of light]] Rather than returning to state 1 given a light change back to OFF, our circuit takes advantage of a memory component to effect a unique progression of states.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image:theNew.jpg|thumb|300px|right|As the light alternates from OFF/ON, our circuit progresses to a third and fourth state!]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> [[Image:theNew.jpg|thumb|300px|right|As the light alternates from OFF/ON, our circuit progresses to a third and fourth state!]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">What are the ramifications of this project? We believe that our circuit design, where progression across sets of protein production is controlled solely by light, has the potential to drastically reduce costs in biological manufacturing practices. Furthermore, we hope our project provides a foundational advancement in circuit design by emphasizing the importance of '''abstraction'''. We think the</ins></div></td></tr>
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</table>Ethan richmanhttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=174943&oldid=prevEthan richman: /* Overview */2010-10-27T06:12:46Z<p><span class="autocomment">Overview</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our project this year attempts to tackle what we see as emerging issue in synthetic biology: the complexity of circuit inputs. [[Image:Manyinput.jpg|thumb|200px|right|A user may have to apply many inputs to achieve a distinct set of outputs]] There is a <del class="diffchange diffchange-inline">growing </del>trend among iGEM projects towards intricate systems; the registry of standard parts is growing quickly and the tools at the disposal of a synthetic biologist are increasing rapidly. While many of these systems rely on an autonomous progression of events in the chasis - say, a cell encounters some environmental stimulus which triggers downstream responses - some require precise user control. This is especially true for projects in the manufacturing area, as often a product is achieved following a progression of steps within the cell. Functioning "behind the scenes" to achieve this sort of controllable progression is the genetic circuit, consisting of plasmids loaded with <del class="diffchange diffchange-inline">promotor </del>and transcription factor pairs. Through a combination of regulators and activators, these circuits achieve distinct "states," or set of functions carried out by the cell<del class="diffchange diffchange-inline">. To move among states, a user provide some specific input to the cells</del>. Whether chemical, heat, or some other environmental change, it is common practice that for each state, a unique input must be applied. With this approach, it is quite clear that an increasingly complicated circuit, with many different <del class="diffchange diffchange-inline">promotors</del>, requires an increasingly intricate set of inputs.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our project this year attempts to tackle what we see as emerging issue in synthetic biology: the complexity of circuit inputs. [[Image:Manyinput.jpg|thumb|200px|right|A user may have to apply many inputs to achieve a distinct set of outputs]] There is a <ins class="diffchange diffchange-inline">developing </ins>trend among iGEM projects towards intricate systems; the registry of standard parts is growing quickly and the tools at the disposal of a synthetic biologist are increasing rapidly. While many of these systems rely on an autonomous progression of events in the chasis - say, a cell encounters some environmental stimulus which triggers downstream responses - some require precise user control. This is especially true for projects in the manufacturing area, as often a product is achieved following a progression of steps within the cell. Functioning "behind the scenes" to achieve this sort of controllable progression is the genetic circuit, consisting of plasmids loaded with <ins class="diffchange diffchange-inline">promoter </ins>and transcription factor pairs. Through a combination of regulators and activators, these circuits achieve distinct "states," or set of functions carried out by the cell. Whether chemical, heat, or some other environmental change, it is common practice that for each state, a unique input must be applied. With this approach, it is quite clear that an increasingly complicated circuit, with many different <ins class="diffchange diffchange-inline">promoters</ins>, requires an increasingly intricate set of inputs.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We believe this to be a problem for a few reasons:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We believe this to be a problem for a few reasons:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#*From an end user perspective, navigation across states could become nightmarish. Especially for a system targeted beyond the lab, perhaps at a non-technical setting, input/control must be accessible and easily attainable.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#*From an end user perspective, navigation across states could become nightmarish. Especially for a system targeted beyond the lab, perhaps at a non-technical setting, input/control must be accessible and easily attainable.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Expense</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div># Expense</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#*Chemical induction of individual components of a circuit, particularly on a large bioreactor scale as in <del class="diffchange diffchange-inline">the manufacturing of ____ </del>, is expensive! For a full fledged, industrial bioreactor, an enormous amount of inducer must be introduced. One chemical input alone on this scale can vastly increase the cost of the end product; multiple chemical inputs drives costs extremely high.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#*Chemical induction of individual components of a circuit, particularly on a large bioreactor scale as in <ins class="diffchange diffchange-inline">telescoping synthesis</ins>, is expensive! For a full<ins class="diffchange diffchange-inline">-</ins>fledged, industrial bioreactor, an enormous amount of inducer must be introduced. One chemical input alone on this scale can vastly increase the cost of the end product; multiple chemical inputs drives costs extremely high.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div># <del class="diffchange diffchange-inline">Interference</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div># <ins class="diffchange diffchange-inline">Efficiency</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#*Environmental changes, such as heat or pH, may remove a cell from optimal conditions for protein production. Again, from a manufacturing standpoint, a decrease in yield per reaction increases costs and decreases overal efficiency.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#*Environmental changes, such as heat or pH, may remove a cell from optimal conditions for protein production. Again, from a manufacturing standpoint, a decrease in yield per reaction increases costs and decreases overal efficiency.</div></td></tr>
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</table>Ethan richmanhttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=174884&oldid=prevTgjohnst: Team:Brown/Project/LRC overview moved to Team:Brown/Project/Light pattern2010-10-27T06:09:00Z<p><a href="/Team:Brown/Project/LRC_overview" class="mw-redirect" title="Team:Brown/Project/LRC overview">Team:Brown/Project/LRC overview</a> moved to <a href="/Team:Brown/Project/Light_pattern" title="Team:Brown/Project/Light pattern">Team:Brown/Project/Light pattern</a></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==Light-Pattern Controlled Circuit<del class="diffchange diffchange-inline">|center</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==Light-Pattern Controlled Circuit==</div></td></tr>
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</table>Ethan richmanhttp://2010.igem.org/wiki/index.php?title=Team:Brown/Project/Light_pattern&diff=168800&oldid=prevEthan richman: /* Light-Pattern Controlled Circuit */2010-10-26T23:31:20Z<p><span class="autocomment">Light-Pattern Controlled Circuit</span></p>
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</table>Ethan richman