Team:Brown/Project

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== '''Light-Pattern Controlled Circuit''' ==
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!align="center"|[[Team:Brown|Home]]
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!align="center"|[[Team:Brown/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Brown Official Team Profile]
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!align="center"|[[Team:Brown/Project|Project]]
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!align="center"|[[Team:Brown/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Brown/Modeling|Modeling]]
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!align="center"|[[Team:Brown/Notebook|Notebook]]
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!align="center"|[[Team:Brown/Safety|Safety]]
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== '''Project 1 - TAT-PTD''' ==
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=== Project Description ===
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'''Abstract'''
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=== Progress ===
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Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, '''we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light.''' With this production method, '''we can link multiple synthesis steps to a single, clean and rapidly scalable input.'''
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<a href="https://2010.igem.org/Team:Brown/Project/Light_pattern/Overview"><img src="https://static.igem.org/mediawiki/2010/thumb/c/c2/LRCpeektacropped.jpg/780px-LRCpeektacropped.jpg" width="500px"></a>
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== '''Project 2 - Quad-state light-activation''' ==
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== E. Cargo ==
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=== Project Description ===
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'''Abstract'''
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=== Progress ===
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We designed a modular Tat-Linker Biobrick that will allow for easy fusion of the Tat-PTD to any other biobricked proteins. Specifically, we aimed to fuse this Tat-Linker in RFC25 format to two bacterial transcription factors, LacI and AraC, with the intent of using the two Tat-TFs as a tool to induce transient gene expression in <i>E. coli</i> without the need to follow through with more time-consuming cell transformation protocols. To test these Tat-TFs, we designed corresponding reporter constructs. We saw a potential application in the portion of the Light-Pattern Controlled Circuit of our project, as well as in any other future genetic circuits that require part-by-part testing.
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== '''Project 3 - Miracle Yogurt''' ==
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<a href="https://2010.igem.org/Team:Brown/Project/Ecargo/Overview"><img src="https://static.igem.org/mediawiki/2010/thumb/8/8d/ECargo.png/400px-ECargo.png" width="500px"></a>
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=== Background ===
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In the early eighteenth century, French cartographer and navigator Chevalier des Marchais travelled all along the west coast of Africa. His maps and manuscripts were published after his death by Père J. B. Labat in Amsterdam in 1730-31. One of these, from 1725, documented a peculiar food culture among local tribes, who consumed tiny red berries before most meals.
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These berries are known as Synsepalum dulcificum, Richadella dulcifica, or colloquially ‘miracle fruit’. They have a peculiar quality: for 30 minutes to two hours after consumption, sour foods are perceived as sweet. This fascinating property was not rediscovered until the mid-19th century, when Prof. Kenzo Kurihara, a Japanese scientist, published an article in Science about the isolation of the active compound, which he coined miraculin.
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Soon afterward, Robert Harvey and Don Emery became co-founders of a miracle berry start-up called Miralin. In 1974, the FDA cut support for the company and halted the approval of miracle berries as a harmless food additive only weeks after the Miralin offices were raided by an unknown party. Although the identity of the thieves was never determined, claims have been made that the raid and subsequent FDA disapproval were supported by high-ups in the sugar and sweetener industry.
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Today, miracle berries are making an underground comeback thanks to a few small startups, with slogans such as mBerry’s “Make life sweeter.” In the last few years, their popularity has grown exponentially, and it’s all thanks to a single protein named miraculin.
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=== Project Description ===
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Latest revision as of 21:07, 27 October 2010


Light-Pattern Controlled Circuit

Abstract

Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light. With this production method, we can link multiple synthesis steps to a single, clean and rapidly scalable input.

E. Cargo

Abstract

We designed a modular Tat-Linker Biobrick that will allow for easy fusion of the Tat-PTD to any other biobricked proteins. Specifically, we aimed to fuse this Tat-Linker in RFC25 format to two bacterial transcription factors, LacI and AraC, with the intent of using the two Tat-TFs as a tool to induce transient gene expression in E. coli without the need to follow through with more time-consuming cell transformation protocols. To test these Tat-TFs, we designed corresponding reporter constructs. We saw a potential application in the portion of the Light-Pattern Controlled Circuit of our project, as well as in any other future genetic circuits that require part-by-part testing.