Latest revision as of 02:47, 28 October 2010

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BioBricks

BioBricks are the central figure of the iGEM competition. It is pretty important to construct functional and well characterized BioBricks in order to avoid problems for other users. Unfortunately we had to handle some problems with BioBricks from the database. Although we created a lot of BioBricks, we therefore focused on creating good, functional and well characterized BioBricks. Our best three BioBricks are listed below. The detailed information of these BioBricks can be received by using the links.

K389004

Luciferase from pGL4.10[luc2]:

Partinfo of the BioBrick <partinfo>K389004</partinfo>

Characterization of K389004

K389015

Final sensor system with luciferase readout:

Partinfo of the BioBrick <partinfo>K389015</partinfo>

Characterization of K389015

K389016

Final sensor system with mRFP readout:

Partinfo of the BioBrick <partinfo>K389016</partinfo>

Characterization of K389016

Correction of faulty BioBricks

Initially, we wanted to use the VirA BioBrick <partinfo>K238008</partinfo> and the virB promoter <partinfo>K238011</partinfo> in our project, but could only obtain unexpected/faulty restriction patterns. Finally we chose to sequence these parts, hoping to find the cause for the maintained restriction patterns. Unfortunately we could not approve the sequences of <partinfo>BBa_K238008</partinfo> and <partinfo>BBa_K238011</partinfo> deposited in the partsregistry so that we chose to design our own VirA and virB promoter BioBricks. We strongly recommend using our VirA receptor and virB promoter since it has been approved by multiple means, e.g. restriction patterns and sequencing (<partinfo>K389001</partinfo> and <partinfo>BBa_K389003</partinfo>).

Improvement of an existing BioBrick

By sending in our mutated virG gene <partinfo>K389002</partinfo> that works in E. coli without the rpoA gene from A. tumefaciens we improved the existing virG BioBrick from the registry <partinfo>K238009</partinfo>.

Characterizing already existing BioBricks

We helped characterizing the following BioBricks:

<partinfo>P1010</partinfo>
<partinfo>I746370</partinfo>
<partinfo>I746380</partinfo>
<partinfo>I746390</partinfo>
<partinfo>K343006</partinfo>

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-= Biobricks =
+= BioBricks =
-Biobricks are the central figure of the iGEM competition. It is pretty important to construct functional Biobricks, in order to avoid problems for other teams. Unfortunately we had to handle some problems with Biobricks from the database. Although we create a lot of Biobricks, we therefore focused on creating good and functional working Biobricks. Our best three Biobricks are listed below. The detailed information of the Biobricks can be received by using the links.
+BioBricks are the central figure of the iGEM competition. It is pretty important to construct functional and well characterized BioBricks in order to avoid problems for other users. Unfortunately we had to handle some problems with BioBricks from the database. Although we created a lot of BioBricks, we therefore focused on creating good, functional and well characterized BioBricks. Our best three BioBricks are listed below. The detailed information of these BioBricks can be received by using the links.
 == K389004 ==
-Luciferase from PGL4.10 [Luc2]:
+Luciferase from pGL4.10[luc2]:
-Partinfo of the Biobrick <partinfo>K389004</partinfo>
+Partinfo of the BioBrick <partinfo>K389004</partinfo>
 [[Team:Bielefeld-Germany/Results/Characterization#BBa_K389004:_Luciferase_from_pGL4.10.5Bluc2.5D| Characterization of K389004]]
@@ Line 33: / Line 34: @@
 == K389015 ==
-Final sensor system with luc readout:
+Final sensor system with luciferase readout:
-Partinfo of the Biobrick <partinfo>K389015</partinfo>
+Partinfo of the BioBrick <partinfo>K389015</partinfo>
 [[Team:Bielefeld-Germany/Results/Characterization#BBa_K389015:_VirA.2FG_reporter_device_with_luciferase| Characterization of K389015]]
 == K389016 ==
@@ Line 43: / Line 45: @@
 Final sensor system with mRFP readout:
-Partinfo of the Biobrick <partinfo>K389016</partinfo>
+Partinfo of the BioBrick <partinfo>K389016</partinfo>
 [[Team:Bielefeld-Germany/Results/Characterization#BBa_K389016:_VirA.2FG_reporter_device_with_mRFP| Characterization of K389016]]
+== Correction of faulty BioBricks ==
+Initially, we wanted to use the VirA BioBrick <partinfo>K238008</partinfo> and the ''virB'' promoter <partinfo>K238011</partinfo> in our project, but could only obtain unexpected/faulty restriction patterns. Finally we chose to sequence these parts, hoping to find the cause for the maintained restriction patterns. Unfortunately we could not approve the sequences of <partinfo>BBa_K238008</partinfo> and <partinfo>BBa_K238011</partinfo> deposited in the partsregistry so that we chose to design our own VirA and ''virB'' promoter BioBricks. We strongly recommend using our VirA receptor and ''virB'' promoter since it has been approved by multiple means, ''e.g.'' restriction patterns and sequencing (<partinfo>K389001</partinfo> and <partinfo>BBa_K389003</partinfo>).
+== Improvement of an existing BioBrick==
+By sending in our mutated ''virG'' gene <partinfo>K389002</partinfo> that works in ''E. coli'' without the ''rpoA'' gene from ''A. tumefaciens'' we improved the existing ''virG'' BioBrick from the registry <partinfo>K238009</partinfo>.
+== Characterizing already existing BioBricks==
+We helped characterizing the following BioBricks:
+* <partinfo>P1010</partinfo>
+* <partinfo>I746370</partinfo>
+* <partinfo>I746380</partinfo>
+* <partinfo>I746390</partinfo>
+* <partinfo>K343006</partinfo>

Team:Bielefeld-Germany/Judging/BioBricks

From 2010.igem.org