Team:Baltimore US/Team

From 2010.igem.org

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(What we did)
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[[Image:TitleBarBalti US.png | center]]
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing. I'd like to have a small paragraph or blurb from the members put together here. with perhaps a caption.
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|[[Image:Baltimore_US_logo.png|200px|right|frame]]
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Title: DIY-GEM: a path towards low cost high throughput gene synthesis. <br>
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Synthetic biology research requires more cost effective approaches <br>
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{| style="background-color:#7998AD;" cellpadding="1" cellspacing="1" border="0" bordercolor="#fff" width="924px" align="center"
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toward reagents and hardware accessibility.  We are developing <br>
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!align="center"|[[Team:Baltimore_US|<span style="color:white;">Home</span>]]
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low-cost alternatives to existing hardware and enzymes in an <br>
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!align="center"|[[Team:Baltimore_US/Team|<span style="color:white;">Team</span>]]
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attempt to expand participation in biological research and <br>
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!align="center"|[http://igem.org/Team.cgi?year=2010&team_name=Baltimore_US <span style="color:white;">Official Team Profile</span>]
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development.Our project expands the accessibility of Taq Polymerase by <br>
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!align="center"|[[Team:Baltimore_US/Project|<span style="color:white;">Project</span>]]
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engineering it in a form compatible with BioBrick assembly.<br>
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!align="center"|[[Team:Baltimore_US/Parts|<span style="color:white;">Submitted Parts</span>]]
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This allows use of the over-expressed enzyme from a crude<br>
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!align="center"|[[Team:Baltimore_US/Modeling|<span style="color:white;">Modeling</span>]]
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bacterial extract in a PCR reaction at a fraction of the cost<br>
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!align="center"|[[Team:Baltimore_US/Notebook|<span style="color:white;">Notebook</span>]]
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of highly purified commercial enzyme.  In addition, we have<br>
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!align="center"|[[Team:Baltimore_US/MeetingTimes|<span style="color:white;">Meeting/Lab Times</span>]]
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developed inexpensive and easily assembled lab equipment such<br>
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!align="center"|[[Team:Baltimore_US/Safety|<span style="color:white;">Safety</span>]]
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as a gel electrophoresis apparatus and a PCR thermal cycler.<br>
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Enabling researchers to synthesize their own enzymes and having<br>
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access to inexpensive tools will allow for increased participation<br>
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among the DIY-bio community, stretch increasingly scarce educational<br>
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funds, and allow rapid scale up of large scale gene synthesis projects.<br>
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{| style= "background-color:#FFFFF;" width="924px" align="center"
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__NOTOC__
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== DIY-GEM: a path towards low cost high throughput gene synthesis. ==
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{|
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|[[Image:Baltimore_US_team.png|400 px]]
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|align="center"|[[Team:Baltimore_US | Team Example]]
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Synthetic biology research requires more cost effective approaches toward reagents and hardware accessibility. We are developing low-cost alternatives to existing hardware and enzymes in an attempt to expand participation in biological research and development. Our project expands the accessibility of Taq Polymerase by engineering it in a form compatible with BioBrick assembly. This allows use of the over-expressed enzyme from a crude bacterial extract in a PCR reaction at a fraction of the cost of highly purified commercial enzyme. In addition, we have developed inexpensive and easily assembled lab equipment such as a gel electrophoresis apparatus and a PCR thermal cycler. Enabling researchers to synthesize their own enzymes and having access to inexpensive tools will allow for increased participation among the DIY-bio community, stretch increasingly scarce educational funds, and allow rapid scale up of large scale gene synthesis projects.<br>
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|[[Image:Baltimore_US_team.png|400px]]
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|}
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="80%" align="center"
 
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!align="center"|[[Team:Baltimore_US|Home]]
 
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!align="center"|[[Team:Baltimore_US/Team|Team]]
 
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!align="center"|[http://igem.org/Team.cgi?year=2010&team_name=Baltimore_US Official Team Profile]
 
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!align="center"|[[Team:Baltimore_US/Project|Project]]
 
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!align="center"|[[Team:Baltimore_US/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Baltimore_US/Modeling|Modeling]]
 
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!align="center"|[[Team:Baltimore_US/Notebook|Notebook]]
 
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!align="center"|[[Team:Baltimore_US/MeetingTimes|Meeting/Lab Times This Week]]
 
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!align="center"|[[Team:Baltimore_US/Safety|Safety]]
 
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|}
 
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== '''Who we are''' ==
== '''Who we are''' ==
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*''' Lab Manager/Advisor''':    Thomas Burkett
*''' Lab Manager/Advisor''':    Thomas Burkett
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*'''Advisor 1''':     Lisa Scheifele
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*'''Advisor 1''': Lisa Scheifele
*'''Advisor 2''':    T Elizabeth Goode   
*'''Advisor 2''':    T Elizabeth Goode   
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'''Undergrads:'''
'''Undergrads:'''
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*'''Student 1''':   Ryan Ogle, LMT  
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*'''Student 1''': Ryan Ogle, LMT  
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*'''Student 2''':   Patrick O'Neil
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*'''Student 2''': Patrick O'Neil
*'''Student 3''':  Robert Buck  
*'''Student 3''':  Robert Buck  
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*'''Student 4''':   Miles Pekala
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*'''Student 4''': Miles Pekala
*'''Student 5''':  Gary Cygiel
*'''Student 5''':  Gary Cygiel
*'''Student 6''':  Steven Stowell
*'''Student 6''':  Steven Stowell
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Image:Patrickmugshot.jpg|<br><br>'''Patrick O'Neill'''  
Image:Patrickmugshot.jpg|<br><br>'''Patrick O'Neill'''  
Image:Baltimore_US_Team_member_3.png|(Robert's mugshot here) <br><br>'''Robert Buck'''  
Image:Baltimore_US_Team_member_3.png|(Robert's mugshot here) <br><br>'''Robert Buck'''  
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Image:Baltimore_US_Team_member_4.png|(Miles' mugshot here) <br><br>'''Miles A. Pekala'''  
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Image:Baltimore_US_Team_member_4.png|(Miles' mugshot here) <br><br>'''Miles Pekala'''  
Image:Baltimore_US_Team_member_5.png|(Gary's mugshot here) <br><br>'''Gary Cygiel'''  
Image:Baltimore_US_Team_member_5.png|(Gary's mugshot here) <br><br>'''Gary Cygiel'''  
Image:Baltimore_US_Team_member_6.png|(Steve's mugshot here) <br><br>'''Steven Stowell'''  
Image:Baltimore_US_Team_member_6.png|(Steve's mugshot here) <br><br>'''Steven Stowell'''  

Revision as of 04:17, 27 October 2010

TitleBarBalti US.png
Home Team Official Team Profile Project Submitted Parts Modeling Notebook Meeting/Lab Times Safety


DIY-GEM: a path towards low cost high throughput gene synthesis.

Synthetic biology research requires more cost effective approaches toward reagents and hardware accessibility. We are developing low-cost alternatives to existing hardware and enzymes in an attempt to expand participation in biological research and development. Our project expands the accessibility of Taq Polymerase by engineering it in a form compatible with BioBrick assembly. This allows use of the over-expressed enzyme from a crude bacterial extract in a PCR reaction at a fraction of the cost of highly purified commercial enzyme. In addition, we have developed inexpensive and easily assembled lab equipment such as a gel electrophoresis apparatus and a PCR thermal cycler. Enabling researchers to synthesize their own enzymes and having access to inexpensive tools will allow for increased participation among the DIY-bio community, stretch increasingly scarce educational funds, and allow rapid scale up of large scale gene synthesis projects.

Baltimore US team.png



Who we are



Advisors:

  • Lab Manager/Advisor: Thomas Burkett
  • Advisor 1: Lisa Scheifele
  • Advisor 2: T Elizabeth Goode


Undergrads:

  • Student 1: Ryan Ogle, LMT
  • Student 2: Patrick O'Neil
  • Student 3: Robert Buck
  • Student 4: Miles Pekala
  • Student 5: Gary Cygiel
  • Student 6: Steven Stowell
  • Student 7: Duke Kessler
  • Student 8: Bernadette Gallagher


What we did

DIYPatrick1.JPG


DIYMiles.JPG


DIYRobert.JPG

Where we're from