Team:Baltimore US/Team

From 2010.igem.org

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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing. I'd like to have a small paragraph or blurb from the members put together here. with perhaps a caption.
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|[[Image:Baltimore_US_logo.png|200px|right|frame]]
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Title: DIY-GEM: a path towards low cost high throughput gene synthesis. <br>
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Synthetic biology research requires more cost effective approaches <br>
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{| style="background-color:#7998AD;" cellpadding="1" cellspacing="1" border="0" bordercolor="#fff" width="924px" align="center"
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toward reagents and hardware accessibility.  We are developing <br>
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!align="center"|[[Team:Baltimore_US|<span style="color:white;">Home</span>]]
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low-cost alternatives to existing hardware and enzymes in an <br>
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!align="center"|[[Team:Baltimore_US/Team|<span style="color:white;">Team</span>]]
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attempt to expand participation in biological research and <br>
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Baltimore_US <span style="color:white;">Official Team Profile</span>]
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development.Our project expands the accessibility of Taq Polymerase by <br>
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!align="center"|[[Team:Baltimore_US/Project|<span style="color:white;">Project</span>]]
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engineering it in a form compatible with BioBrick assembly.<br>
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!align="center"|[[Team:Baltimore_US/Parts|<span style="color:white;">Submitted Parts</span>]]
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This allows use of the over-expressed enzyme from a crude<br>
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!align="center"|[[Team:Baltimore_US/Modeling|<span style="color:white;">Modeling</span>]]
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bacterial extract in a PCR reaction at a fraction of the cost<br>
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!align="center"|[[Team:Baltimore_US/Notebook|<span style="color:white;">Notebook</span>]]
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of highly purified commercial enzyme.  In addition, we have<br>
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!align="center"|[[Team:Baltimore_US/MeetingTimes|<span style="color:white;">Meeting/Lab Times</span>]]
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developed inexpensive and easily assembled lab equipment such<br>
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!align="center"|[[Team:Baltimore_US/Safety|<span style="color:white;">Safety</span>]]
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as a gel electrophoresis apparatus and a PCR thermal cycler.<br>
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Enabling researchers to synthesize their own enzymes and having<br>
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access to inexpensive tools will allow for increased participation<br>
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among the DIY-bio community, stretch increasingly scarce educational<br>
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funds, and allow rapid scale up of large scale gene synthesis projects.<br>
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{| style= "background-color:#FFFFF;" width="924px" align="center"
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__NOTOC__
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== DIY-GEM: a path towards low cost high throughput gene synthesis. ==
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{|
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|[[Image:Baltimore_US_team.png|400 px]]
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|align="center"|[[Team:Baltimore_US | Team Example]]
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Synthetic biology research requires more cost effective approaches toward reagents and hardware accessibility. We are developing low-cost alternatives to existing hardware and enzymes in an attempt to expand participation in biological research and development. Our project expands the accessibility of Taq Polymerase by engineering it in a form compatible with BioBrick assembly. This allows use of the over-expressed enzyme from a crude bacterial extract in a PCR reaction at a fraction of the cost of highly purified commercial enzyme. In addition, we have developed inexpensive and easily assembled lab equipment such as a gel electrophoresis apparatus and a PCR thermal cycler. Enabling researchers to synthesize their own enzymes and having access to inexpensive tools will allow for increased participation among the DIY-bio community, stretch increasingly scarce educational funds, and allow rapid scale up of large scale gene synthesis projects.<br>
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|[[Image:Baltimore_US_team.png|400px]]
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|}
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="80%" align="center"
 
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!align="center"|[[Team:Baltimore_US|Home]]
 
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!align="center"|[[Team:Baltimore_US/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Baltimore_US Official Team Profile]
 
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!align="center"|[[Team:Baltimore_US/Project|Project]]
 
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!align="center"|[[Team:Baltimore_US/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Baltimore_US/Modeling|Modeling]]
 
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!align="center"|[[Team:Baltimore_US/Notebook|Notebook]]
 
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!align="center"|[[Team:Baltimore_US/MeetingTimes|Meeting/Lab Times This Week]]
 
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!align="center"|[[Team:Baltimore_US/Safety|Safety]]
 
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|}
 
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== '''Who we are''' ==
== '''Who we are''' ==
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*''' Lab Manager/Advisor''':    Thomas Burkett
*''' Lab Manager/Advisor''':    Thomas Burkett
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*'''Advisor 1''':     Lisa Scheifele
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*'''Advisor 1''': Lisa Scheifele
*'''Advisor 2''':    T Elizabeth Goode   
*'''Advisor 2''':    T Elizabeth Goode   
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'''Undergrads:'''
'''Undergrads:'''
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*'''Student 1''':   Ryan Ogle, LMT  
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*'''Student 1''': Ryan Ogle, LMT  
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*'''Student 2''':   Patrick O'Neil
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*'''Student 2''': Patrick O'Neil
*'''Student 3''':  Robert Buck  
*'''Student 3''':  Robert Buck  
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*'''Student 4''':   Miles Pekala
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*'''Student 4''': Miles Pekala
*'''Student 5''':  Gary Cygiel
*'''Student 5''':  Gary Cygiel
*'''Student 6''':  Steven Stowell
*'''Student 6''':  Steven Stowell
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Image:Patrickmugshot.jpg|<br><br>'''Patrick O'Neill'''  
Image:Patrickmugshot.jpg|<br><br>'''Patrick O'Neill'''  
Image:Baltimore_US_Team_member_3.png|(Robert's mugshot here) <br><br>'''Robert Buck'''  
Image:Baltimore_US_Team_member_3.png|(Robert's mugshot here) <br><br>'''Robert Buck'''  
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Image:Baltimore_US_Team_member_4.png|(Miles' mugshot here) <br><br>'''Miles A. Pekala'''  
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Image:Baltimore_US_Team_member_4.png|(Miles' mugshot here) <br><br>'''Miles Pekala'''  
Image:Baltimore_US_Team_member_5.png|(Gary's mugshot here) <br><br>'''Gary Cygiel'''  
Image:Baltimore_US_Team_member_5.png|(Gary's mugshot here) <br><br>'''Gary Cygiel'''  
Image:Baltimore_US_Team_member_6.png|(Steve's mugshot here) <br><br>'''Steven Stowell'''  
Image:Baltimore_US_Team_member_6.png|(Steve's mugshot here) <br><br>'''Steven Stowell'''  

Revision as of 04:17, 27 October 2010

TitleBarBalti US.png
Home Team Official Team Profile Project Submitted Parts Modeling Notebook Meeting/Lab Times Safety


DIY-GEM: a path towards low cost high throughput gene synthesis.

Synthetic biology research requires more cost effective approaches toward reagents and hardware accessibility. We are developing low-cost alternatives to existing hardware and enzymes in an attempt to expand participation in biological research and development. Our project expands the accessibility of Taq Polymerase by engineering it in a form compatible with BioBrick assembly. This allows use of the over-expressed enzyme from a crude bacterial extract in a PCR reaction at a fraction of the cost of highly purified commercial enzyme. In addition, we have developed inexpensive and easily assembled lab equipment such as a gel electrophoresis apparatus and a PCR thermal cycler. Enabling researchers to synthesize their own enzymes and having access to inexpensive tools will allow for increased participation among the DIY-bio community, stretch increasingly scarce educational funds, and allow rapid scale up of large scale gene synthesis projects.

Baltimore US team.png



Who we are



Advisors:

  • Lab Manager/Advisor: Thomas Burkett
  • Advisor 1: Lisa Scheifele
  • Advisor 2: T Elizabeth Goode


Undergrads:

  • Student 1: Ryan Ogle, LMT
  • Student 2: Patrick O'Neil
  • Student 3: Robert Buck
  • Student 4: Miles Pekala
  • Student 5: Gary Cygiel
  • Student 6: Steven Stowell
  • Student 7: Duke Kessler
  • Student 8: Bernadette Gallagher


Go Team Venture!

Ryan Ogle, LMT



Patrick O'Neill

Baltimore US Team member 3.png

(Robert's mugshot here)

Robert Buck

Baltimore US Team member 4.png

(Miles' mugshot here)

Miles Pekala

Baltimore US Team member 5.png

(Gary's mugshot here)

Gary Cygiel

Baltimore US Team member 6.png

(Steve's mugshot here)

Steven Stowell

Baltimore US Team member 7.png

(Duke's mugshot here)

Duke Kessler

(Bernadette's mugshot here)
Bernadette Gallagher

Baltimore US Team member 9.png

(Tom's mugshot here)

Tom Burkett - Lab Manager/Advisor & Contact Person

Baltimore US Team member 10.png

(Lisa's mugshot here)

Lisa Scheifele - Advisor

Baltimore US Team member 11.png

(Liz's mugshot here )

Liz Goode - Advisor

What we did

DIYPatrick1.JPG


DIYMiles.JPG


DIYRobert.JPG

Where we're from

Retrieved from "http://2010.igem.org/Team:Baltimore_US/Team"