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Team:Baltimore US/Notebook
From 2010.igem.org
BioMechanic (Talk | contribs) (Lab Update 6-7-10) |
(described patrick's work on 6/7) |
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The legend for the various parts is as follows... | The legend for the various parts is as follows... | ||
| + | <table> | ||
| + | <tr><td>1PO</td><td>BBa_R0063</td></tr> | ||
| + | <tr><td>2PO</td><td>BBa_P0412</td></tr> | ||
| + | <tr><td>3PO</td><td>pSBIT3</td></tr> | ||
| + | </table> | ||
| - | + | Patrick cut parts BBa_r0063 and BBa_p0412, and the plasmid backbone pSBIT3. The next step is to ligate them. | |
| - | + | ||
Revision as of 00:53, 8 June 2010
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Notebook
June 7, 2010
Tom greeted us with 4 separate sheets that contained 3 reactions each for us to begin restrictions and began heating to let the enzymes cut. A lesson in what not to do, was offered as we began the heating cycle of restriction in the PCR blocks and he timing had been set to 35 seconds instead of 35 minutes, after which it heated to a kill cycle of 80 degrees and we had to reapply the enzymes, in case the enzymes had been denatured.
We had 6 individuals building the 4 sheets, 2 in redundancy.
The legend for the various parts is as follows...
| 1PO | BBa_R0063 |
| 2PO | BBa_P0412 |
| 3PO | pSBIT3 |
Patrick cut parts BBa_r0063 and BBa_p0412, and the plasmid backbone pSBIT3. The next step is to ligate them.
