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Team:BIOTEC Dresden/Protocols:Electroporation - Revision history
2024-03-28T14:12:43Z
Revision history for this page on the wiki
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Lucas.schirmer: Removing all content from page
2010-09-13T17:34:18Z
<p>Removing all content from page</p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:34, 13 September 2010</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{{Biotec_Dresden/Header}}</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><html></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><body></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><h2>Materials</h2></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Escherichia coli DH5 alpha electrocompetent cells</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Plasmid DNA</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Ice</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>2mm gap width Electroporation cuvette</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Electroporater</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>1.5ml microfuge tube</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>1mL SOC (room temperature) for each reaction</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>LB-agar plate with corresponding antibiotic</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><h2>Procedure</h2></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Chill electroporation cuvettes, DNA samples and tubes on ice.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Place LB-agar plates in 37&deg;C incubator twarm.<li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Once cuvettes are cold, remove electrocompetent cells from -80&deg;C freezer and thaw on ice. Alternatively, freshly prepared electrocompetent cells may be used immediately.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>If electrocompetent cells are not already in individual aliquots, then aliquot out intpre-chilled 0.6mL tubes.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Turn on electroporator and set voltage teither 1.25 kV (1mm cuvettes) or 2.5 kV (2mm cuvettes).</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Dial a P2 pipetman teither 1 or 2µL depending on the salt content of your DNA sample and . Use 2µL for samples that have been purified in some way.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Dial a P200 pipetman t50µL or whatever volume of electrocompetent cells you want tuse. Usually 20-50µL.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Dial a P1000 pipetman t950µL and pipet in SOC. Place pipetman on counter such that tip doesn't touch anything.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Pipet 1-2µL of DNA sample and add telectrocompetent cells. Swirl tip around gently in cells tmix DNA and cells. Dnot pipet up and down. </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Place cells back on ice tensure they remain cold.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Transfer cell-DNA mixture tcuvettes using P200 pipetman. Try not thandle cuvette base tomuch sthat it stays cold.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Tap the cuvette on the counter gently sthat cells are at the bottom and tremove any air bubbles. </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Wipe off excess moisture from outside of cuvette.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Place in chamber of electroporator.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Slide the chamber in sthat the cuvette sits snugly between electrodes.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Pulse the cells with a shock by pressing button on electroporator.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Remove cuvette from the chamber and immediately add SOC. This step should be done as quickly as possible tprevent cells from dying off.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Transfer SOC-cell mixture tchilled eppendorf tube.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Chill sample on ice for 2 mins tpermit the cells trecover.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Transfer eppendorf tube t37&deg;C incubator and shake tpromote aeration. Incubate for 1 hr tpermit expression of antibiotic resistance gene. </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Plate transformation ontprewarmed LB-agar plate supplemented with appropriate antibiotic. I generally plate 200µL but appropriate plating volume depends on efficiency of the transformation.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> Incubate plate overnight at 37&deg;C.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> Leave remaining SOC-cell mixture on the benchtop overnight.</li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> If you don't have any transformants, plate the rest of the transformation in the morning.</li></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Category:BIOTEC Dresden/Protocol]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{{Biotec_Dresden/Bottom}}</del></div></td><td colspan="2"> </td></tr>
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69974&oldid=prev
Lucas.schirmer at 16:36, 13 September 2010
2010-09-13T16:36:59Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 16:36, 13 September 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Header}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Header}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">script type="text</del>/<del class="diffchange diffchange-inline">JavaScript"</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">body></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> $</del>(<del class="diffchange diffchange-inline">'</del>.<del class="diffchange diffchange-inline">mw</del>-<del class="diffchange diffchange-inline">normal</del>-<del class="diffchange diffchange-inline">catlinks'</del>).<del class="diffchange diffchange-inline">hide</del>();</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><h2>Materials<</ins>/<ins class="diffchange diffchange-inline">h2</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div></<del class="diffchange diffchange-inline">script</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ul></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Escherichia coli DH5 alpha electrocompetent cells</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Plasmid DNA</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Ice</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>2mm gap width Electroporation cuvette</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Electroporater</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>1.5ml microfuge tube</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>1mL SOC </ins>(<ins class="diffchange diffchange-inline">room temperature) for each reaction</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>LB-agar plate with corresponding antibiotic</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></ul></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><h2>Procedure</h2></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ul></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Chill electroporation cuvettes, DNA samples and tubes on ice</ins>.<ins class="diffchange diffchange-inline"></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Place LB</ins>-<ins class="diffchange diffchange-inline">agar plates in 37&deg;C incubator twarm.<li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Once cuvettes are cold, remove electrocompetent cells from </ins>-<ins class="diffchange diffchange-inline">80&deg;C freezer and thaw on ice. Alternatively, freshly prepared electrocompetent cells may be used immediately.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>If electrocompetent cells are not already in individual aliquots, then aliquot out intpre-chilled 0.6mL tubes.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Turn on electroporator and set voltage teither 1.25 kV (1mm cuvettes</ins>) <ins class="diffchange diffchange-inline">or 2</ins>.<ins class="diffchange diffchange-inline">5 kV </ins>(<ins class="diffchange diffchange-inline">2mm cuvettes</ins>)<ins class="diffchange diffchange-inline">.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Dial a P2 pipetman teither 1 or 2µL depending on the salt content of your DNA sample and . Use 2µL for samples that have been purified in some way.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Dial a P200 pipetman t50µL or whatever volume of electrocompetent cells you want tuse. Usually 20-50µL.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Dial a P1000 pipetman t950µL and pipet in SOC. Place pipetman on counter such that tip doesn't touch anything.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Pipet 1-2µL of DNA sample and add telectrocompetent cells. Swirl tip around gently in cells tmix DNA and cells. Dnot pipet up and down. </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Place cells back on ice tensure they remain cold.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Transfer cell-DNA mixture tcuvettes using P200 pipetman. Try not thandle cuvette base tomuch sthat it stays cold.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Tap the cuvette on the counter gently sthat cells are at the bottom and tremove any air bubbles. </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Wipe off excess moisture from outside of cuvette.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Place in chamber of electroporator.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Slide the chamber in sthat the cuvette sits snugly between electrodes.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Pulse the cells with a shock by pressing button on electroporator.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Remove cuvette from the chamber and immediately add SOC. This step should be done as quickly as possible tprevent cells from dying off.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Transfer SOC-cell mixture tchilled eppendorf tube.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Chill sample on ice for 2 mins tpermit the cells trecover.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Transfer eppendorf tube t37&deg</ins>;<ins class="diffchange diffchange-inline">C incubator and shake tpromote aeration. Incubate for 1 hr tpermit expression of antibiotic resistance gene. </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Plate transformation ontprewarmed LB-agar plate supplemented with appropriate antibiotic. I generally plate 200µL but appropriate plating volume depends on efficiency of the transformation.</ins></<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li> Incubate plate overnight at 37&deg;C.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li> Leave remaining SOC-cell mixture on the benchtop overnight.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li> If you don't have any transformants, plate the rest of the transformation in the morning.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></ul></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></body></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC Dresden/Protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC Dresden/Protocol]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td></tr>
</table>
Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69960&oldid=prev
Lucas.schirmer at 16:26, 13 September 2010
2010-09-13T16:26:45Z
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69958&oldid=prev
Lucas.schirmer at 16:25, 13 September 2010
2010-09-13T16:25:57Z
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69957&oldid=prev
Lucas.schirmer at 16:25, 13 September 2010
2010-09-13T16:25:24Z
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69955&oldid=prev
Lucas.schirmer at 16:24, 13 September 2010
2010-09-13T16:24:53Z
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69954&oldid=prev
Lucas.schirmer at 16:24, 13 September 2010
2010-09-13T16:24:37Z
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69953&oldid=prev
Lucas.schirmer at 16:23, 13 September 2010
2010-09-13T16:23:36Z
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69952&oldid=prev
Lucas.schirmer at 16:23, 13 September 2010
2010-09-13T16:23:14Z
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Lucas.schirmer
http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Protocols:Electroporation&diff=69951&oldid=prev
Lucas.schirmer at 16:22, 13 September 2010
2010-09-13T16:22:45Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC Dresden/Protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC Dresden/Protocol]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td></tr>
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Lucas.schirmer