http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&feed=atom&action=historyTeam:BIOTEC Dresden/Characterized Parts/BBa K407013 - Revision history2024-03-29T02:01:57ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=207755&oldid=prevLucas.schirmer at 03:35, 28 October 20102010-10-28T03:35:45Z<p></p>
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</table>Lucas.schirmerhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=206926&oldid=prevLucas.schirmer at 03:16, 28 October 20102010-10-28T03:16:10Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="caption"><p>Figure 1: The fluorescence of YFP is shown over increasing</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="caption"><p>Figure 1: The fluorescence of YFP is shown over increasing</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p> concentrations of AHL after 2 hours of incubation</p></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p> concentrations of AHL after 2 hours of incubation</p></div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td></tr>
</table>Lucas.schirmerhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=206662&oldid=prevRahul at 03:09, 28 October 20102010-10-28T03:09:13Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Materials and methods</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Materials and methods</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The characterization was performed using a 96-well plate and a fluorescence plate reader, which was kept at 37°C during the whole measurement. Bacteria supplied with the part <del class="diffchange diffchange-inline">BBa_ </del>were suspended in medium of a certain concentration of AHL of 500 nM and 2000nM. The fluorescence was measured every 5 minutes using an excitation wavelength of 485nm and an emission wavelength of 535nm. For every fluorescence value, also the optical density at 612nm was measured. As a negative control, the same measurements were done on uninduced bacteria and LB-medium without cells.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The characterization was performed using a 96-well plate and a fluorescence plate reader, which was kept at 37°C during the whole measurement. Bacteria supplied with the part <ins class="diffchange diffchange-inline">BBa_407013 </ins>were suspended in medium of a certain concentration of AHL of 500 nM and 2000nM. The fluorescence was measured every 5 minutes using an excitation wavelength of 485nm and an emission wavelength of 535nm. For every fluorescence value, also the optical density at 612nm was measured. As a negative control, the same measurements were done on uninduced bacteria and LB-medium without cells.</p></div></td></tr>
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</table>Rahulhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=206584&oldid=prevLucas.schirmer at 03:06, 28 October 20102010-10-28T03:06:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Objective of part assembly</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Objective of part assembly</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>To detect the amount of AHL present in the extracellular environment, different detection systems were built which assure a high sensitivity. For this purpose existing parts from the registry were assembled.</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>To detect the amount of AHL present in the extracellular environment, different detection systems were built which assure a high sensitivity. For this purpose existing parts from the registry were assembled.</p> </div></td></tr>
</table>Lucas.schirmerhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=205892&oldid=prevSarah Mansour at 02:45, 28 October 20102010-10-28T02:45:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki <a href="https://static.igem.org/mediawiki/igem.org/7/75/Protocol_AHLassay.pdf"> here. </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki <a href="https://static.igem.org/mediawiki/igem.org/7/75/Protocol_AHLassay.pdf"> here. </a></p></div></td></tr>
</table>Sarah Mansourhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=205791&oldid=prevSarah Mansour at 02:41, 28 October 20102010-10-28T02:41:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki <a href="https://static.igem.org/mediawiki/igem.org/7/75/Protocol_AHLassay.pdf"> here. </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki <a href="https://static.igem.org/mediawiki/igem.org/7/75/Protocol_AHLassay.pdf"> here. </a></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC_Dresden/Characterized_Parts|K407013]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC_Dresden/Characterized_Parts|K407013]]</div></td></tr>
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</table>Sarah Mansourhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=205517&oldid=prevSarah Mansour at 02:27, 28 October 20102010-10-28T02:27:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki. <del class="diffchange diffchange-inline">(Link)</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki <ins class="diffchange diffchange-inline"><a href="https://static</ins>.<ins class="diffchange diffchange-inline">igem.org/mediawiki/igem.org/7/75/Protocol_AHLassay.pdf"> here. </a></ins></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></body> </html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></body> </html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC_Dresden/Characterized_Parts|K407013]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:BIOTEC_Dresden/Characterized_Parts|K407013]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td></tr>
</table>Sarah Mansourhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=205316&oldid=prevRahul at 02:21, 28 October 20102010-10-28T02:21:00Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:21, 28 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The characterization was performed using a 96-well plate and a fluorescence plate reader, which was kept at 37°C during the whole measurement. Bacteria supplied with the part BBa_ were suspended in medium of a certain concentration of AHL of 500 nM and 2000nM. The fluorescence was measured every 5 minutes using an excitation wavelength of 485nm and an emission wavelength of 535nm. For every fluorescence value, also the optical density at 612nm was measured. As a negative control, the same measurements were done on uninduced bacteria and LB-medium without cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The characterization was performed using a 96-well plate and a fluorescence plate reader, which was kept at 37°C during the whole measurement. Bacteria supplied with the part BBa_ were suspended in medium of a certain concentration of AHL of 500 nM and 2000nM. The fluorescence was measured every 5 minutes using an excitation wavelength of 485nm and an emission wavelength of 535nm. For every fluorescence value, also the optical density at 612nm was measured. As a negative control, the same measurements were done on uninduced bacteria and LB-medium without cells.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div class="border left"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/c/ce/Ptet_yfp.png"><img src="https://static.igem.org/mediawiki/2010/c/ce/Ptet_yfp.png" class="border thumb center"></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/c/ce/Ptet_yfp.png"><img src="https://static.igem.org/mediawiki/2010/c/ce/Ptet_yfp.png" class="border thumb center"></a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The above figure shows the plot between the Raw Fluorescence Units and Time in minutes for the Lux receiver which has a YFP output when induced by different AHL concentrations such as 500 nM and 2000 nM. From the graph, it is evident that there is an increasing fluorescence signal with increase in time of measurement. In case of higher levels of AHL concentration, the signal output is also seen on the higher scale. The LB medium has a fluorescent signal, which remains almost constant during the measurement. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div class="caption"><p>Figure 1: The fluorescence of YFP is shown over increasing</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p> concentrations of AHL after 2 hours of incubation</p></div></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></div></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The above figure shows the plot between the Raw Fluorescence Units and Time in minutes for the Lux receiver which has a YFP output when induced by different AHL concentrations such as 500 nM and 2000 nM. From the graph, it is evident that there is an increasing fluorescence signal with increase in time of measurement. In case of higher levels of AHL concentration, the signal output is also seen on the higher scale. The LB medium has a fluorescent signal, which remains almost constant during the measurement.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Only two concentrations, viz. 500 Nm and 2000 nM of AHL were tested for induction of the cells which does not totally quantify the data which we have. Hence, it would be useful to test the same part at really low concentrations of AHL and determine the level of fluorescence output received. At zero concentration of AHL, a slight amount of fluorescence was detected which might be due to one of the following reasons: (i) The part was tested in a plasmid backbone containing ampicillin wherein there were no stop codons at the end of the construct. (ii) The promoter might have been leaky. This holds further scope as it would make sense to test the part in chloramphenicol backbone so as to strongly repress the constitutive expression of the reporter protein.</p></div></td></tr>
</table>Rahulhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=204806&oldid=prevRahul at 02:06, 28 October 20102010-10-28T02:06:52Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:06, 28 October 2010</td>
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<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The characterization was performed using a 96-well plate and a fluorescence plate reader, which was kept at 37°C during the whole measurement. Bacteria supplied with the part BBa_ were suspended in medium of a certain concentration of AHL of 500 nM and 2000nM. The fluorescence was measured every 5 minutes using an excitation wavelength of 485nm and an emission wavelength of 535nm. For every fluorescence value, also the optical density at 612nm was measured. As a negative control, the same measurements were done on uninduced bacteria and LB-medium without cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The characterization was performed using a 96-well plate and a fluorescence plate reader, which was kept at 37°C during the whole measurement. Bacteria supplied with the part BBa_ were suspended in medium of a certain concentration of AHL of 500 nM and 2000nM. The fluorescence was measured every 5 minutes using an excitation wavelength of 485nm and an emission wavelength of 535nm. For every fluorescence value, also the optical density at 612nm was measured. As a negative control, the same measurements were done on uninduced bacteria and LB-medium without cells.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="https://static.igem.org/mediawiki/2010/c/ce/Ptet_yfp.png"><img src="https://static.igem.org/mediawiki/2010/c/ce/Ptet_yfp.png" class="border thumb center"></a></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The above figure shows the plot between the Raw Fluorescence Units and Time in minutes for the Lux receiver which has a YFP output when induced by different AHL concentrations such as 500 nM and 2000 nM. From the graph, it is evident that there is an increasing fluorescence signal with increase in time of measurement. In case of higher levels of AHL concentration, the signal output is also seen on the higher scale. The LB medium has a fluorescent signal, which remains almost constant during the measurement. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The above figure shows the plot between the Raw Fluorescence Units and Time in minutes for the Lux receiver which has a YFP output when induced by different AHL concentrations such as 500 nM and 2000 nM. From the graph, it is evident that there is an increasing fluorescence signal with increase in time of measurement. In case of higher levels of AHL concentration, the signal output is also seen on the higher scale. The LB medium has a fluorescent signal, which remains almost constant during the measurement. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Discussion</h2></div></td></tr>
</table>Rahulhttp://2010.igem.org/wiki/index.php?title=Team:BIOTEC_Dresden/Characterized_Parts/BBa_K407013&diff=204536&oldid=prevRahul at 01:56, 28 October 20102010-10-28T01:56:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Additional information</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki. (Link)</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>For detailed information of the experimental set-up, please have a look at the protocols on our team-wiki. (Link)</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:BIOTEC_Dresden/Characterized_Parts|K407013]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{Biotec_Dresden/Bottom}}</ins></div></td></tr>
</table>Rahul