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Team:BIOTEC Dresden/Characterized Parts/BBa K407008
From 2010.igem.org
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| - | + | <h2>Objective of part assembly</h2> | |
<p>In order to determine the kinetics and toxicity of the reporter proteins, ecfp and eyfp, constructs constitutively expressing both of them were assembled. Secondly, these fluorescent reporters are important tools to further improve and investigate our method of normalization like performed with RFP.(link) Depending on the desired application and output signal, ecfp as well as eyfp can be coupled to our reporter systems described in the first characterized part. (link)</p> | <p>In order to determine the kinetics and toxicity of the reporter proteins, ecfp and eyfp, constructs constitutively expressing both of them were assembled. Secondly, these fluorescent reporters are important tools to further improve and investigate our method of normalization like performed with RFP.(link) Depending on the desired application and output signal, ecfp as well as eyfp can be coupled to our reporter systems described in the first characterized part. (link)</p> | ||
This part is meant to serve as a device that constitutively expresses the cyan fluorescent protein (CFP) by means of the promoter ptetR while repressing the same in the presence of tetracycline. Hence, tetracycline acts as a negative regulator here. | This part is meant to serve as a device that constitutively expresses the cyan fluorescent protein (CFP) by means of the promoter ptetR while repressing the same in the presence of tetracycline. Hence, tetracycline acts as a negative regulator here. | ||
Revision as of 00:14, 28 October 2010
Objective of part assembly
In order to determine the kinetics and toxicity of the reporter proteins, ecfp and eyfp, constructs constitutively expressing both of them were assembled. Secondly, these fluorescent reporters are important tools to further improve and investigate our method of normalization like performed with RFP.(link) Depending on the desired application and output signal, ecfp as well as eyfp can be coupled to our reporter systems described in the first characterized part. (link)
This part is meant to serve as a device that constitutively expresses the cyan fluorescent protein (CFP) by means of the promoter ptetR while repressing the same in the presence of tetracycline. Hence, tetracycline acts as a negative regulator here.
