"
Team:BCCS-Bristol/Wetlab/Part Design/BioBricks
From 2010.igem.org
(New page: {{:Team:BCCS-Bristol/Header}} ==BioBricks== Pages for the two biobricks that we've submitted, why we had to make them, improvements, characterisation, link to them in the registry [[:Te...) |
(→BioBricks) |
||
| Line 8: | Line 8: | ||
[[Team:BCCS-Bristol/Wetlab/Part Design/BioBricks/PyeaR+LacZ]] | [[Team:BCCS-Bristol/Wetlab/Part Design/BioBricks/PyeaR+LacZ]] | ||
| + | |||
| + | |||
| + | The PyeaR promoter was first put into the BioBrick standard by the University of Edinburgh’s | ||
| + | 2009 iGEM team. We used this promoter and connected it to a GFP BioBrick to create a BioBrick | ||
| + | that expresses GFP in response to levels of nitrite. We also connected the promoter to the LacZ | ||
| + | gene which codes for β -galactosidase. This was used in a Miller assay, an experiment that is used | ||
| + | to characterise a repressors response to inducer. | ||
| + | |||
| + | |||
| + | The constitutive RFP was expressed via an unmodified BioBrick from the parts registry. | ||
Latest revision as of 13:53, 13 October 2010
iGEM 2010
BioBricks
Pages for the two biobricks that we've submitted, why we had to make them, improvements, characterisation, link to them in the registry
Team:BCCS-Bristol/Wetlab/Part Design/BioBricks/PyeaR
Team:BCCS-Bristol/Wetlab/Part Design/BioBricks/PyeaR+LacZ
The PyeaR promoter was first put into the BioBrick standard by the University of Edinburgh’s
2009 iGEM team. We used this promoter and connected it to a GFP BioBrick to create a BioBrick
that expresses GFP in response to levels of nitrite. We also connected the promoter to the LacZ
gene which codes for β -galactosidase. This was used in a Miller assay, an experiment that is used
to characterise a repressors response to inducer.
The constitutive RFP was expressed via an unmodified BioBrick from the parts registry.
