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Team:BCCS-Bristol/Wetlab/K381001 Construction
From 2010.igem.org
Having decided on our biobrick design, our first task was to aquire the necessary parts and construct our first biobrick.
BBa_I13522 was taken from well 8A kitplate 2 of the 2010 distribution BBa_K216005 was requested from the parts registry
Construction of the biobrick can be broadly broken down into
- Generating larger quantities of both our component biobricks
- Digesting each of these biobricks
- Ligating together the desired parts to create a new biobrick
- Generating larger quantities of this biobrick, checking and sequencing
