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From 2010.igem.org

Contents 1 Improvements 1.1 Improving the system 1.1.1 Adding further promoters 1.1.2 Increasing the difference in signal levels 1.2 Improving the beads 1.2.1 Adding a coating 1.2.2 Adding LB

Improvements

We’re extremely proud of what we’ve accomplished this year on the wetlab side; first building and well characterising our biobrick, but then also developing our novel bead approach to improving signal detection. However the project is still far from perfect, below are several things we would like to implement were we to take the project further.

Improving the system

Adding further promoters

The most obvious extension to the project is to add the ability to detect multiple nutrients. In terms of improving fertiliser use, it would make most sense to add phosphate and potassium detection, as together with nitrogen these make up the major components of fertiliser.

There are already promoters available in the parts registry that sense Phosphate (K116401 submitted by NYMU-Taipei). What’s more, the wide variety of fluorescent proteins available in the parts registry makes it easy to add another signal, without requiring different detection technology. These additional signals would also be compatible with our ratio method of calibrating signals.

Increasing the difference in signal levels

Improving the beads

Adding a coating

Adding LB