Team:BCCS-Bristol/Wetlab/Beads

From 2010.igem.org

(Difference between revisions)
Line 17: Line 17:
-
Initial soil visibility experiments (see [http://2010.igem.org/Team:BCCS-Bristol/Wetlab/signal_soil here]) raised the major problem that high levels of cell death when ''E. coli'' were spread freely in the soil resulted in very low signals. This isn't surprising, given lab-grade ''E. coli'' are deliberately synthesised such that they can't survive outside of very specific conditions, and are easily outcompeted by naturally occuring soil bacteria.
+
Initial soil visibility experiments (see [http://2010.igem.org/Team:BCCS-Bristol/Wetlab/signal_soil here]) raised the major problem that high levels of cell death when ''E. coli'' were spread freely in the soil resulted in very low signals. This isn't surprising, given lab-grade ''E. coli'' are deliberately synthesised such that they can't survive outside of very specific conditions, and are easily out competed by naturally occurring soil bacteria.
Line 25: Line 25:
* Large concentrations of cells in a small area ensure high, easily detectable signals.
* Large concentrations of cells in a small area ensure high, easily detectable signals.
* Nutrients can be added to beads, further improving bacteria survival.
* Nutrients can be added to beads, further improving bacteria survival.
-
* Bacteria are kept seperate from the environment, reducing public safety fears.
+
* Bacteria are kept separate from the environment, reducing public safety fears.
* Increased access to Nitrate.
* Increased access to Nitrate.
Line 33: Line 33:
-
We hope our work on bacterial encapsulation will prove useful to teams in the future - as encapsulation has a number of applications beyond our soil sensor (and has considerable safety benefits) - so have provided below information on the material we chose to use, and the exact procedure for making beads. Above you can find links materials and procedures, as well as experiments analysing the performance of beads containing our new biobrick, and a gallery of bead images.
+
We hope our work on bacterial encapsulation will prove useful to teams in the future - as encapsulation has a number of applications beyond our soil sensor (and has considerable safety benefits) - so have provided below information on the material we chose to use, and the exact procedure for making beads. Above you can find links materials and procedures, as well as experiments analysing the performance of beads containing our new BioBrick, and a gallery of bead images.

Revision as of 18:09, 27 October 2010

OverviewBead MaterialsMaking BeadsBeads in SolutionBeads in SoilImage Gallery

Beads

One of the factors that differentiate us from other teams is our use of technology to facilitate using our bacteria safely and more effectively in real life situations. More specifically; we encapsulated our bacteria in a non-toxic gel substances, forming beads of highly concentrated bacteria.


Why did we do this?

Our new beads


Initial soil visibility experiments (see here) raised the major problem that high levels of cell death when E. coli were spread freely in the soil resulted in very low signals. This isn't surprising, given lab-grade E. coli are deliberately synthesised such that they can't survive outside of very specific conditions, and are easily out competed by naturally occurring soil bacteria.


Whilst developed to solve this problem, our beads have a number of beneficial features:

  • Beads provide a protective environment, reducing cell death.
  • Large concentrations of cells in a small area ensure high, easily detectable signals.
  • Nutrients can be added to beads, further improving bacteria survival.
  • Bacteria are kept separate from the environment, reducing public safety fears.
  • Increased access to Nitrate.


More Information


We hope our work on bacterial encapsulation will prove useful to teams in the future - as encapsulation has a number of applications beyond our soil sensor (and has considerable safety benefits) - so have provided below information on the material we chose to use, and the exact procedure for making beads. Above you can find links materials and procedures, as well as experiments analysing the performance of beads containing our new BioBrick, and a gallery of bead images.