Team:BCCS-Bristol/Wetlab

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Wet lab
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== Wet lab ==
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'''09/07/2010 - Day One'''
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Made two different competent strains of ''E.coli'', and attempted transformations on both using BBa_I13522 (pTet GFP) from the kit - prepared 6 plates: 1 positive control for each strain, 1 negative control for each strain and 1 test transformation plate for each strain. The positive control was carried out using a plasmid of known quantity. Ampicillin was used for selection.
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'''12/07/2010 - Day Two'''
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No growth on test plates.

Revision as of 10:14, 19 July 2010

Wet lab

09/07/2010 - Day One

Made two different competent strains of E.coli, and attempted transformations on both using BBa_I13522 (pTet GFP) from the kit - prepared 6 plates: 1 positive control for each strain, 1 negative control for each strain and 1 test transformation plate for each strain. The positive control was carried out using a plasmid of known quantity. Ampicillin was used for selection.

12/07/2010 - Day Two

No growth on test plates.